Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 92461..92996 Replicon plasmid unnamed3
Accession NZ_CP018073
Organism Enterococcus faecium strain VRE001

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag BOW68_RS16940 Protein ID WP_002305108.1
Coordinates 92461..92733 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag BOW68_RS16945 Protein ID WP_002303154.1
Coordinates 92733..92996 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOW68_RS16910 87467..88654 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
BOW68_RS16915 88842..89789 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
BOW68_RS16920 90086..90634 - 549 WP_002292681.1 hypothetical protein -
BOW68_RS16925 90635..91492 - 858 WP_002292680.1 AAA family ATPase -
BOW68_RS16930 91778..92065 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
BOW68_RS16935 92055..92384 - 330 WP_002292678.1 AbrB family transcriptional regulator -
BOW68_RS16940 92461..92733 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
BOW68_RS16945 92733..92996 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BOW68_RS16950 93102..93518 - 417 WP_002303153.1 hypothetical protein -
BOW68_RS16955 93524..94204 - 681 WP_010729614.1 IS6 family transposase -
BOW68_RS16960 94745..95935 - 1191 WP_002314359.1 IS256 family transposase -
BOW68_RS16965 96676..97035 + 360 WP_073120210.1 iron-sulfur cluster biosynthesis family protein -
BOW68_RS16970 97108..97926 - 819 WP_002303335.1 Cof-type HAD-IIB family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..170854 170854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T68665 WP_002305108.1 NZ_CP018073:c92733-92461 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T68665 NZ_CP018073:c92733-92461 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT68665 WP_002303154.1 NZ_CP018073:c92996-92733 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT68665 NZ_CP018073:c92996-92733 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References