Detailed information of TA system TA068596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1225214..1225775
• Replicon: chromosome
• Accession: NZ_CP018034
• Organism: Glaesserella parasuis str. Nagasaki

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMA9
• Locus tag: A2U21_RS06550
• Protein ID: WP_010786777.1
• Coordinates: 1225214..1225492 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: A2U21_RS06555
• Protein ID: WP_005712616.1
• Coordinates: 1225503..1225775 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U21_RS06535	1220997..1222325	-	1329	WP_021109502.1	exodeoxyribonuclease VII large subunit	-
A2U21_RS06540	1222439..1223701	-	1263	WP_021109501.1	serine hydroxymethyltransferase	-
A2U21_RS06545	1224055..1225065	-	1011	WP_021109500.1	ornithine carbamoyltransferase	-
A2U21_RS06550	1225214..1225492	+	279	WP_010786777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A2U21_RS06555	1225503..1225775	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
A2U21_RS06560	1225839..1227059	+	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
A2U21_RS06565	1227069..1227377	+	309	Protein_1234	DUF853 family protein	-
A2U21_RS06570	1227449..1228663	-	1215	WP_021109499.1	ATP-binding protein	-
A2U21_RS06575	1228871..1229389	-	519	WP_012621700.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10278.66 Da
• Isoelectric Point: 10.0505

>T68596 WP_010786777.1 NZ_CP018034:1225214-1225492 [Glaesserella parasuis str. Nagasaki]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKVQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T68596 NZ_CP018034:1225214-1225492 [Glaesserella parasuis str. Nagasaki]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACTGCAGGTATTCAGGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTGCAAGGTAATCTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAAGTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT68596 WP_005712616.1 NZ_CP018034:1225503-1225775 [Glaesserella parasuis str. Nagasaki]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT68596 NZ_CP018034:1225503-1225775 [Glaesserella parasuis str. Nagasaki]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGCGTCACTCGTGTCACGCTTTCTCGTATTTTAAATGGTAAAGCTGCAATTTCACCTGAAATGGCTCTTAGAT
TGAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: U4SMA9