Detailed information of TA system TA068587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1360526..1361169
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: A2U20_RS06705
• Protein ID: WP_005714197.1
• Coordinates: 1360825..1361169 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A2U20_RS06700
• Protein ID: WP_016527809.1
• Coordinates: 1360526..1360822 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS06675	1355675..1356514	-	840	WP_021117038.1	sir2 family protein	-
A2U20_RS06680	1356541..1357377	-	837	WP_021117039.1	sir2 family protein	-
A2U20_RS06685	1357455..1358165	-	711	WP_021117040.1	NAD-dependent protein deacylase	-
A2U20_RS06690	1358176..1358919	-	744	WP_021117041.1	protein phosphatase 2C domain-containing protein	-
A2U20_RS06695	1359501..1360463	-	963	WP_021117042.1	WYL domain-containing protein	-
A2U20_RS06700	1360526..1360822	-	297	WP_016527809.1	helix-turn-helix domain-containing protein	Antitoxin
A2U20_RS06705	1360825..1361169	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A2U20_RS06710	1361384..1363363	+	1980	WP_021117043.1	exoribonuclease II	-
A2U20_RS06715	1363474..1364565	+	1092	WP_021109715.1	murein transglycosylase A	-
A2U20_RS06720	1364747..1365343	-	597	WP_021117044.1	DedA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1304438..1373191	68753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T68587 WP_005714197.1 NZ_CP018032:c1361169-1360825 [Glaesserella parasuis D74]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T68587 NZ_CP018032:c1361169-1360825 [Glaesserella parasuis D74]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCCCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11196.11 Da
• Isoelectric Point: 8.8246

>AT68587 WP_016527809.1 NZ_CP018032:c1360822-1360526 [Glaesserella parasuis D74]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPELIPVLQGL

Nucleotide

• Length: 297 bp

>AT68587 NZ_CP018032:c1360822-1360526 [Glaesserella parasuis D74]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCACATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCTCTTTTAGGCGTAAGCCAAAATCTTGTGCAGTCGTGGGAAATCGGCAGACGAACCCCAAATGGTGCGGCACGGAAA
TTACTTGCGATTATTGAGCAACAGCCAGAACTTATCCCTGTATTACAAGGGCTTTAA

Structures

Toxin

• AlphaFold DB: U4SMB1