Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1127586..1128147 Replicon chromosome
Accession NZ_CP018032
Organism Glaesserella parasuis D74

Toxin (Protein)


Gene name higB Uniprot ID U4SMA9
Locus tag A2U20_RS05470 Protein ID WP_010786777.1
Coordinates 1127869..1128147 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag A2U20_RS05465 Protein ID WP_005712616.1
Coordinates 1127586..1127858 (-) Length 91 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2U20_RS05450 1124109..1124651 + 543 WP_021115958.1 single-stranded DNA-binding protein -
A2U20_RS05455 1124805..1126292 - 1488 WP_021116872.1 DUF853 domain-containing protein -
A2U20_RS05460 1126302..1127522 - 1221 WP_021116873.1 ATP-dependent RNA helicase RhlB -
A2U20_RS05465 1127586..1127858 - 273 WP_005712616.1 HigA family addiction module antidote protein Antitoxin
A2U20_RS05470 1127869..1128147 - 279 WP_010786777.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A2U20_RS05475 1128296..1129306 + 1011 WP_021116874.1 ornithine carbamoyltransferase -
A2U20_RS11970 1129412..1129573 - 162 WP_021116875.1 hypothetical protein -
A2U20_RS05480 1129660..1130001 - 342 WP_043895690.1 hypothetical protein -
A2U20_RS05485 1130092..1130688 - 597 WP_021116877.1 hypothetical protein -
A2U20_RS05490 1131027..1132289 + 1263 WP_021116878.1 serine hydroxymethyltransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-92)

Antitoxin

(1-81)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10278.66 Da        Isoelectric Point: 10.0505

>T68585 WP_010786777.1 NZ_CP018032:c1128147-1127869 [Glaesserella parasuis D74]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKVQN
GHAEVVDYQDYH

Download         Length: 279 bp

>T68585 NZ_CP018032:c1128147-1127869 [Glaesserella parasuis D74]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACTGCAGGTATTCAGGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATCTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAAGTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10314.06 Da        Isoelectric Point: 9.8982

>AT68585 WP_005712616.1 NZ_CP018032:c1127858-1127586 [Glaesserella parasuis D74]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Download         Length: 273 bp

>AT68585 NZ_CP018032:c1127858-1127586 [Glaesserella parasuis D74]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTTTCTCGTATTTTAAATGGCAAAGCTGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U4SMA9


Antitoxin

Source ID Structure

References