Detailed information of TA system TA068583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 516633..517283
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SB00
• Locus tag: A2U20_RS12110
• Protein ID: WP_021117806.1
• Coordinates: 516945..517283 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A2U20_RS12105
• Protein ID: WP_021117805.1
• Coordinates: 516633..516941 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS02720	513064..513543	-	480	WP_021117804.1	transcription elongation factor GreB	-
A2U20_RS02725	513543..514478	-	936	WP_005710401.1	KpsF/GutQ family sugar-phosphate isomerase	-
A2U20_RS02730	514539..515120	-	582	WP_021110392.1	type IV pilus biogenesis/stability protein PilW	-
A2U20_RS02735	515272..516432	-	1161	WP_010786053.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
A2U20_RS12105	516633..516941	-	309	WP_021117805.1	helix-turn-helix domain-containing protein	Antitoxin
A2U20_RS12110	516945..517283	-	339	WP_021117806.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A2U20_RS02770	518119..519558	+	1440	WP_043895984.1	glutamate--tRNA ligase	-
A2U20_RS02780	520025..520291	-	267	Protein_515	cation-transporting ATPase PacS	-
A2U20_RS02785	520246..521179	-	934	Protein_516	transposase	-
A2U20_RS02790	521300..521674	-	375	WP_021111694.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	520640..521179	539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-86)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13202.18 Da
• Isoelectric Point: 9.8649

>T68583 WP_021117806.1 NZ_CP018032:c517283-516945 [Glaesserella parasuis D74]
MNVAFVELPPFEEYRKKYLDDDSFRLLQNELLKFPDKGELIQGTGGLRKLRIADIIRQKGKRGGARVIYYYYVQGKQVWL
FHAYNKNQQDDLSNEERVVLANTLSYLKSLVR

Nucleotide

• Length: 339 bp

>T68583 NZ_CP018032:c517283-516945 [Glaesserella parasuis D74]
ATGAATGTCGCTTTTGTGGAGTTGCCGCCTTTTGAGGAATATAGAAAAAAGTATCTTGACGATGACTCATTTAGATTATT
ACAAAATGAGTTACTAAAATTTCCCGATAAGGGAGAGCTTATTCAAGGCACTGGAGGACTAAGAAAATTACGTATTGCGG
ATATTATTAGACAAAAAGGAAAACGAGGCGGAGCTAGGGTAATCTATTACTATTATGTACAAGGGAAACAAGTTTGGTTG
TTCCATGCCTATAATAAAAACCAACAAGACGATTTATCTAATGAAGAACGTGTGGTTTTGGCGAATACATTAAGCTATTT
AAAATCTTTAGTGAGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11553.68 Da
• Isoelectric Point: 10.4294

>AT68583 WP_021117805.1 NZ_CP018032:c516941-516633 [Glaesserella parasuis D74]
MGKLFNELMEGMQALELHLKGKITLKTTVVEIPDPIVITPEEVKAIRQKLNLSQAVFARKLHTSVRTYQGWEQGRTKPNP
QAVLLLKMVEKSPKTFEQIASI

Nucleotide

• Length: 309 bp

>AT68583 NZ_CP018032:c516941-516633 [Glaesserella parasuis D74]
ATGGGAAAATTATTTAATGAACTAATGGAAGGGATGCAAGCATTAGAGTTGCATTTAAAAGGCAAAATCACATTAAAAAC
CACTGTCGTAGAAATTCCGGATCCGATAGTAATTACCCCTGAAGAAGTGAAAGCTATTCGCCAAAAATTAAATCTTTCAC
AAGCGGTTTTTGCTCGTAAATTACACACTTCGGTGAGAACTTATCAAGGTTGGGAGCAAGGAAGAACAAAGCCCAATCCG
CAAGCGGTCTTACTATTAAAAATGGTTGAAAAATCGCCAAAAACATTTGAGCAAATTGCGAGCATTTAA

Structures

Toxin

• AlphaFold DB: U4SB00