Detailed information of TA system TA068548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5470713..5471455
• Replicon: chromosome
• Accession: NZ_CP018016
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Y71_RS26290
• Protein ID: WP_035942746.1
• Coordinates: 5471144..5471455 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y71_RS26285
• Protein ID: WP_007372402.1
• Coordinates: 5470713..5471147 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS26275	5468294..5469643	+	1350	WP_007372404.1	lysine-sensitive aspartokinase 3	-
Y71_RS26280	5469737..5470678	+	942	WP_007372403.1	ketopantoate/pantoate/pantothenate transporter PanS	-
Y71_RS26285	5470713..5471147	-	435	WP_007372402.1	helix-turn-helix domain-containing protein	Antitoxin
Y71_RS26290	5471144..5471455	-	312	WP_035942746.1	type II toxin-antitoxin system HigB family toxin	Toxin
Y71_RS26295	5471705..5471977	+	273	WP_007372400.1	DUF3811 domain-containing protein	-
Y71_RS26300	5471978..5472841	-	864	WP_007372399.1	23S rRNA pseudouridine(2604) synthase RluF	-
Y71_RS26305	5472976..5474607	-	1632	WP_007372398.1	Na/Pi cotransporter family protein	-
Y71_RS26310	5474782..5475258	-	477	WP_139201288.1	hypothetical protein	-
Y71_RS26315	5475233..5476009	-	777	WP_035942742.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (23-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12272.11 Da
• Isoelectric Point: 9.9087

>T68548 WP_035942746.1 NZ_CP018016:c5471455-5471144 [Kosakonia radicincitans DSM 16656]
MHVISRRPFDSAANDFPNHALVLDAVYKVLKSTSYRNPDELREEFPSLDRMKYRDKWWVINIAGNHLRMLFFADFGAGKI
FVKHIVTHAEYDKLVKRYRETKE

Nucleotide

• Length: 312 bp

>T68548 NZ_CP018016:c5471455-5471144 [Kosakonia radicincitans DSM 16656]
ATGCACGTCATATCAAGGAGGCCGTTTGATAGTGCGGCCAACGATTTTCCAAACCATGCCCTTGTGCTGGATGCTGTTTA
CAAGGTGCTGAAAAGTACATCTTACCGCAATCCTGATGAATTGAGAGAAGAGTTCCCCAGCCTGGACAGAATGAAATACC
GGGATAAATGGTGGGTTATCAATATCGCGGGCAATCATCTGAGAATGCTGTTTTTTGCGGATTTCGGGGCGGGAAAAATT
TTCGTGAAGCACATTGTGACGCATGCTGAGTATGACAAACTGGTGAAGCGTTACCGGGAGACTAAAGAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16013.09 Da
• Isoelectric Point: 4.5542

>AT68548 WP_007372402.1 NZ_CP018016:c5471147-5470713 [Kosakonia radicincitans DSM 16656]
MIDDAIKAADQLVKQVPFLGNRPSREDYEHALAMVEQLLEQAPDSPLVELLAAKIERYEDNDPELVAFNTRVASLPRGVA
ALRVLMDQYGLNQSSFGHEIGQRSLVSRILNGERNLTVDHIRALAERFNVSTDVFIEPVSQTAG

Nucleotide

• Length: 435 bp

>AT68548 NZ_CP018016:c5471147-5470713 [Kosakonia radicincitans DSM 16656]
ATGATTGATGATGCAATAAAGGCAGCCGATCAACTGGTGAAACAGGTCCCTTTTCTGGGGAATCGCCCCAGCAGAGAGGA
CTACGAACACGCGCTGGCGATGGTTGAACAATTGCTTGAGCAGGCTCCGGATAGTCCACTGGTCGAACTACTTGCGGCGA
AAATAGAACGCTATGAGGATAATGATCCGGAACTTGTGGCATTTAATACACGCGTGGCGTCACTGCCACGAGGTGTCGCC
GCCCTGCGCGTTTTGATGGATCAGTATGGATTAAATCAATCTTCCTTTGGCCATGAAATTGGTCAGCGTTCTCTGGTGAG
CCGTATTCTCAATGGCGAACGTAACCTGACGGTCGATCATATTCGGGCTCTGGCTGAGCGATTTAATGTTTCAACTGATG
TATTTATTGAGCCCGTTAGCCAGACGGCAGGTTAA