Detailed information of TA system TA068541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3762514..3763180
• Replicon: chromosome
• Accession: NZ_CP018016
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Y71_RS18195
• Protein ID: WP_007374095.1
• Coordinates: 3762878..3763180 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y71_RS18190
• Protein ID: WP_007374096.1
• Coordinates: 3762514..3762855 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS18155	3757540..3757863	-	324	WP_007374103.1	heavy metal-binding domain-containing protein	-
Y71_RS18160	3757964..3758479	+	516	WP_007374102.1	lipoprotein	-
Y71_RS18165	3758688..3759416	+	729	WP_007374101.1	arginine ABC transporter ATP-binding protein ArtP	-
Y71_RS18170	3759436..3760167	+	732	WP_007374100.1	arginine ABC transporter substrate-binding protein	-
Y71_RS18175	3760174..3760890	+	717	WP_007374099.1	arginine ABC transporter permease ArtQ	-
Y71_RS18180	3760890..3761558	+	669	WP_007374098.1	arginine ABC transporter permease ArtM	-
Y71_RS18185	3761729..3762463	+	735	WP_007374097.1	ABC transporter substrate-binding protein ArtJ	-
Y71_RS18190	3762514..3762855	-	342	WP_007374096.1	HigA family addiction module antidote protein	Antitoxin
Y71_RS18195	3762878..3763180	-	303	WP_007374095.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y71_RS18200	3763246..3764373	-	1128	WP_007374094.1	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC	-
Y71_RS18205	3764415..3764834	-	420	WP_090474329.1	YbjO family protein	-
Y71_RS18210	3764951..3765805	-	855	WP_007374092.1	putrescine ABC transporter permease PotI	-
Y71_RS18215	3765802..3766755	-	954	WP_007374091.1	putrescine ABC transporter permease PotH	-
Y71_RS18220	3766765..3767898	-	1134	WP_007374090.1	putrescine ABC transporter ATP-binding subunit PotG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11877.61 Da
• Isoelectric Point: 10.2331

>T68541 WP_007374095.1 NZ_CP018016:c3763180-3762878 [Kosakonia radicincitans DSM 16656]
MPKKISIRYFRDAWLDEFFHHTTPHRKIPADIHTTLARKLDIINAATSHRDLRSPPGNRYEELSGKLLGYSSIRVNKQYR
LIFRWVNGKADDLYLDPHIY

Nucleotide

• Length: 303 bp

>T68541 NZ_CP018016:c3763180-3762878 [Kosakonia radicincitans DSM 16656]
ATGCCAAAGAAAATAAGCATCAGATATTTTCGGGATGCATGGCTTGATGAATTCTTTCATCACACCACGCCGCACCGGAA
AATTCCGGCCGATATTCACACTACGCTGGCCCGCAAGCTGGATATCATTAATGCCGCGACGTCTCACCGTGATCTTCGTT
CGCCGCCGGGCAATCGCTATGAAGAGTTATCGGGGAAACTGCTCGGTTATTCTTCCATCAGGGTTAATAAGCAGTACCGG
TTAATTTTCAGATGGGTTAACGGAAAGGCGGATGACCTCTATCTGGATCCGCATATCTATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12928.75 Da
• Isoelectric Point: 6.4777

>AT68541 WP_007374096.1 NZ_CP018016:c3762855-3762514 [Kosakonia radicincitans DSM 16656]
MKQATRKPTTVGDILLYEYLEPLELRINELAEILHVHRNSVSALVNNNRRLTAEMAFRLAKAFDTSADFWLNLQAAVDLW
EVENNMRAQEELSRIESAANFIARREAGTKKVA

Nucleotide

• Length: 342 bp

>AT68541 NZ_CP018016:c3762855-3762514 [Kosakonia radicincitans DSM 16656]
ATGAAACAGGCGACCAGAAAGCCAACAACGGTGGGCGACATCCTGCTGTATGAGTACCTGGAACCTCTTGAGCTCAGGAT
TAACGAGCTGGCGGAGATATTGCATGTGCATCGTAACTCCGTCAGTGCGTTGGTTAATAACAACCGGCGGCTGACGGCGG
AGATGGCCTTTCGTCTCGCCAAAGCGTTTGACACATCCGCTGATTTCTGGCTCAACCTGCAAGCGGCCGTCGATTTATGG
GAAGTTGAGAATAATATGCGAGCCCAGGAAGAGCTGAGCCGGATTGAATCGGCGGCGAATTTTATTGCTCGCCGTGAGGC
AGGGACAAAAAAAGTCGCATAA