Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3880508..3881165 Replicon chromosome
Accession NZ_CP017990
Organism Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)


Gene name higB Uniprot ID A0A1Z3MZ87
Locus tag WM95_RS19155 Protein ID WP_063408809.1
Coordinates 3880508..3880807 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MZ32
Locus tag WM95_RS19160 Protein ID WP_063408808.1
Coordinates 3880818..3881165 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM95_RS19145 3876890..3879088 + 2199 WP_023308901.1 type I secretion system permease/ATPase -
WM95_RS19150 3879099..3880268 + 1170 WP_088545052.1 HlyD family efflux transporter periplasmic adaptor subunit -
WM95_RS19155 3880508..3880807 + 300 WP_063408809.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WM95_RS19160 3880818..3881165 + 348 WP_063408808.1 HigA family addiction module antidote protein Antitoxin
WM95_RS19165 3881186..3881704 - 519 WP_063408807.1 hypothetical protein -
WM95_RS19170 3881790..3882971 - 1182 WP_088544959.1 PLP-dependent aminotransferase family protein -
WM95_RS19175 3882992..3883879 - 888 WP_063408805.1 LysR family transcriptional regulator -
WM95_RS19180 3883977..3884579 + 603 WP_023308908.1 short chain dehydrogenase -
WM95_RS19185 3884573..3885307 - 735 WP_063408804.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3753106..3883123 130017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11707.32 Da        Isoelectric Point: 10.0346

>T68487 WP_063408809.1 NZ_CP017990:3880508-3880807 [Enterobacter cloacae complex sp. ECNIH7]
MISYFRDQWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T68487 NZ_CP017990:3880508-3880807 [Enterobacter cloacae complex sp. ECNIH7]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATCCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGTAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCCATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTGTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13039.98 Da        Isoelectric Point: 7.0134

>AT68487 WP_063408808.1 NZ_CP017990:3880818-3881165 [Enterobacter cloacae complex sp. ECNIH7]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT68487 NZ_CP017990:3880818-3881165 [Enterobacter cloacae complex sp. ECNIH7]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAGCTGGCAAAAGCCTTTGATACCACGATTGAATTCTGGCTGAACTTACAGCTGAACGTTGAT
ATCTGGGAAGCGCAATCTAACGCCAGGACGCAGGAGGAACTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAACCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MZ87

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MZ32

References