Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 46842..47499 Replicon plasmid unnamed2
Accession NZ_CP017987
Organism Klebsiella pneumoniae strain 825795-1

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag BB783_RS29295 Protein ID WP_000270043.1
Coordinates 47149..47499 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BB783_RS29290 Protein ID WP_000124640.1
Coordinates 46842..47144 (-) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB783_RS29240 42467..42895 + 429 WP_000591074.1 hypothetical protein -
BB783_RS29245 42952..43311 + 360 WP_000422768.1 hypothetical protein -
BB783_RS29250 43311..43757 + 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
BB783_RS29255 43754..44272 + 519 WP_000210756.1 nitrite reductase -
BB783_RS29260 44272..44502 + 231 WP_000972663.1 hypothetical protein -
BB783_RS29265 44489..45346 + 858 WP_001167032.1 hypothetical protein -
BB783_RS29275 45577..46104 + 528 WP_001236377.1 thermonuclease family protein -
BB783_RS29280 46162..46434 + 273 WP_001043047.1 HU family DNA-binding protein -
BB783_RS29285 46522..46815 + 294 WP_001239997.1 chromosome segregation protein ParM -
BB783_RS29290 46842..47144 - 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
BB783_RS29295 47149..47499 - 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB783_RS29300 47662..48210 + 549 WP_001061574.1 transcriptional regulator -
BB783_RS29310 48551..48745 + 195 WP_000343597.1 hypothetical protein -
BB783_RS29315 48756..49127 + 372 WP_000516916.1 hypothetical protein -
BB783_RS29320 49120..49590 + 471 WP_001281821.1 hypothetical protein -
BB783_RS29325 49605..49940 - 336 WP_000683476.1 hypothetical protein -
BB783_RS29330 50037..50525 + 489 WP_001273096.1 DUF1643 domain-containing protein -
BB783_RS29335 50528..51025 + 498 WP_000062185.1 hypothetical protein -
BB783_RS29345 51240..51944 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
BB783_RS29350 52009..52188 - 180 Protein_68 helix-turn-helix transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / qacE / sul1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaOXA-48 - 1..117168 117168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T68471 WP_000270043.1 NZ_CP017987:c47499-47149 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T68471 NZ_CP017987:c47499-47149 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT68471 WP_000124640.1 NZ_CP017987:c47144-46842 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT68471 NZ_CP017987:c47144-46842 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References