Detailed information of TA system TA068446

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4478958..4479370
• Replicon: chromosome
• Accession: NZ_CP017979
• Organism: Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: BL257_RS23725
• Protein ID: WP_000132601.1
• Coordinates: 4478958..4479299 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4479294..4479370 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL257_RS23715 (BL257_22185)	4476371..4477417	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
BL257_RS23720 (BL257_22190)	4477417..4478796	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
BL257_RS23725 (BL257_22195)	4478958..4479299	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	4479294..4479370	+	77	-	-	Antitoxin
BL257_RS23730 (BL257_22200)	4479527..4480921	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
BL257_RS23735 (BL257_22205)	4480918..4482507	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4471873..4480921	9048

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T68446 WP_000132601.1 NZ_CP017979:c4479299-4478958 [Escherichia coli str. K-12 substr. W3110]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T68446 NZ_CP017979:c4479299-4478958 [Escherichia coli str. K-12 substr. W3110]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT68446 NZ_CP017979:4479294-4479370 [Escherichia coli str. K-12 substr. W3110]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin