Detailed information of TA system TA068440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3058651..3059344
• Replicon: chromosome
• Accession: NZ_CP017979
• Organism: Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: BL257_RS16195
• Protein ID: WP_000415584.1
• Coordinates: 3059048..3059344 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: BL257_RS16190
• Protein ID: WP_000650107.1
• Coordinates: 3058651..3059046 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL257_RS16180 (BL257_15170)	3054515..3056773	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
BL257_RS16185 (BL257_15175)	3056911..3058518	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
BL257_RS16190 (BL257_15180)	3058651..3059046	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
BL257_RS16195 (BL257_15185)	3059048..3059344	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
BL257_RS16200 (BL257_15190)	3059549..3060031	-	483	WP_000183505.1	transcriptional regulator	-
BL257_RS16205 (BL257_15195)	3060084..3060476	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BL257_RS16210 (BL257_15200)	3060628..3061287	+	660	WP_001221495.1	two-component system response regulator QseB	-
BL257_RS16215 (BL257_15205)	3061284..3062633	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
BL257_RS16220 (BL257_15210)	3062679..3063011	-	333	WP_000917684.1	DUF2645 family protein	-
BL257_RS16225 (BL257_15215)	3063330..3063911	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BL257_RS16230 (BL257_15220)	3063942..3064256	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T68440 WP_000415584.1 NZ_CP017979:c3059344-3059048 [Escherichia coli str. K-12 substr. W3110]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T68440 NZ_CP017979:c3059344-3059048 [Escherichia coli str. K-12 substr. W3110]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT68440 WP_000650107.1 NZ_CP017979:c3059046-3058651 [Escherichia coli str. K-12 substr. W3110]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT68440 NZ_CP017979:c3059046-3058651 [Escherichia coli str. K-12 substr. W3110]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65