Detailed information of TA system TA068413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5442709..5443304
• Replicon: chromosome
• Accession: NZ_CP017969
• Organism: Pseudomonas aeruginosa isolate B10W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: BMR72_RS26275
• Protein ID: WP_003113526.1
• Coordinates: 5443026..5443304 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BMR72_RS26270
• Protein ID: WP_003133769.1
• Coordinates: 5442709..5443014 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMR72_RS26235	5437731..5438021	-	291	WP_031757220.1	DUF5447 family protein	-
BMR72_RS26240	5438197..5438469	-	273	WP_004352675.1	hypothetical protein	-
BMR72_RS26250	5438909..5439244	+	336	WP_023094905.1	hypothetical protein	-
BMR72_RS32670	5439338..5439928	+	591	WP_071536478.1	SLATT domain-containing protein	-
BMR72_RS26260	5439925..5441190	+	1266	WP_023094906.1	nucleotidyltransferase	-
BMR72_RS26265	5441274..5442266	-	993	WP_003133766.1	hypothetical protein	-
BMR72_RS26270	5442709..5443014	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
BMR72_RS26275	5443026..5443304	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BMR72_RS26285	5443633..5445861	+	2229	WP_003133778.1	TonB-dependent receptor	-
BMR72_RS26290	5445932..5446579	-	648	WP_003095021.1	carbonate dehydratase	-
BMR72_RS26295	5446641..5447879	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T68413 WP_003113526.1 NZ_CP017969:c5443304-5443026 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T68413 NZ_CP017969:c5443304-5443026 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT68413 WP_003133769.1 NZ_CP017969:c5443014-5442709 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT68413 NZ_CP017969:c5443014-5442709 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3