Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1111903..1112822 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag BK419_RS21120 Protein ID WP_003900449.1
Coordinates 1111903..1112508 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag BK419_RS05315 Protein ID WP_003410124.1
Coordinates 1112517..1112822 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS05290 1109391..1110287 + 897 WP_003410136.1 hypothetical protein -
BK419_RS05295 1110494..1110730 + 237 WP_003410133.1 hypothetical protein -
BK419_RS05300 1110682..1111359 + 678 WP_003905725.1 hypothetical protein -
BK419_RS05305 1111519..1111878 + 360 WP_003410131.1 hypothetical protein -
BK419_RS21120 1111903..1112508 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BK419_RS05315 1112517..1112822 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
BK419_RS05320 1112907..1113206 + 300 WP_003410120.1 hypothetical protein -
BK419_RS05325 1113222..1113638 - 417 WP_003410114.1 hypothetical protein -
BK419_RS05330 1113628..1114347 - 720 WP_003410108.1 DUF433 domain-containing protein -
BK419_RS05335 1114589..1115629 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
BK419_RS05340 1115626..1116003 - 378 Protein_1043 hypothetical protein -
BK419_RS05350 1116054..1117315 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BK419_RS05355 1117359..1117559 - 201 Protein_1045 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T68300 WP_003900449.1 NZ_CP017920:1111903-1112508 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T68300 NZ_CP017920:1111903-1112508 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT68300 WP_003410124.1 NZ_CP017920:1112517-1112822 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT68300 NZ_CP017920:1112517-1112822 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References