Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2656375..2656979 Replicon chromosome
Accession NZ_CP017886
Organism Pseudomonas frederiksbergensis strain ERDD5:01

Toxin (Protein)


Gene name higB Uniprot ID A0A1J0EKU3
Locus tag BLL42_RS12885 Protein ID WP_071552441.1
Coordinates 2656375..2656689 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID J2MYF8
Locus tag BLL42_RS12890 Protein ID WP_007949192.1
Coordinates 2656692..2656979 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLL42_RS12855 2651853..2652341 + 489 WP_071552435.1 hypothetical protein -
BLL42_RS12860 2652436..2652870 - 435 WP_071552436.1 acyl-CoA thioesterase -
BLL42_RS12865 2652919..2654865 - 1947 WP_071552437.1 RecQ family ATP-dependent DNA helicase -
BLL42_RS12875 2655308..2655589 + 282 WP_071552439.1 hypothetical protein -
BLL42_RS12880 2655949..2656245 + 297 WP_071552440.1 hypothetical protein -
BLL42_RS12885 2656375..2656689 + 315 WP_071552441.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BLL42_RS12890 2656692..2656979 + 288 WP_007949192.1 helix-turn-helix domain-containing protein Antitoxin
BLL42_RS12895 2657492..2657698 + 207 WP_071552442.1 YhfG family protein -
BLL42_RS12900 2657730..2658083 + 354 WP_071552443.1 DUF1493 family protein -
BLL42_RS12905 2658127..2658981 + 855 WP_071555751.1 EamA family transporter -
BLL42_RS12910 2659059..2660600 - 1542 WP_071552444.1 phosphoenolpyruvate carboxykinase -
BLL42_RS12920 2660781..2661683 - 903 WP_071552446.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(42-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11682.58 Da        Isoelectric Point: 10.0249

>T68260 WP_071552441.1 NZ_CP017886:2656375-2656689 [Pseudomonas frederiksbergensis]
MIFIETPIFTRRAKELLDDDAYIALQKVLVSNPSAGDVIEGTAGIRKMRVAAKGHGKRGGARVIYYHFVSASQIALLMIY
PKNEQQDLTADERKALKAVIEHWR

Download         Length: 315 bp

>T68260 NZ_CP017886:2656375-2656689 [Pseudomonas frederiksbergensis]
ATGATTTTCATTGAGACCCCAATCTTCACCCGTCGAGCCAAAGAACTATTGGATGACGATGCCTATATCGCCTTGCAGAA
GGTACTGGTGTCGAATCCCTCGGCTGGCGACGTCATCGAAGGGACAGCCGGGATTCGCAAAATGCGGGTCGCGGCCAAGG
GGCACGGCAAACGAGGAGGAGCCCGAGTGATTTACTACCATTTCGTCTCGGCATCGCAGATCGCTTTGTTGATGATCTAC
CCTAAAAATGAGCAACAAGACCTAACCGCCGATGAACGCAAAGCGTTGAAAGCCGTCATCGAACACTGGAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10678.30 Da        Isoelectric Point: 7.3210

>AT68260 WP_007949192.1 NZ_CP017886:2656692-2656979 [Pseudomonas frederiksbergensis]
MSKFFDELMESVQQMDEIVRGERLPSREFHVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAG

Download         Length: 288 bp

>AT68260 NZ_CP017886:2656692-2656979 [Pseudomonas frederiksbergensis]
ATGAGCAAGTTTTTTGATGAGCTGATGGAAAGCGTGCAACAGATGGACGAGATCGTACGGGGTGAACGCCTGCCTTCCCG
CGAATTCCATGTTGACGCATTGCAAGTCAAAGAAATTCGTAAGGCCACCGGTCTGACTCAGGCCAAATTCGCTGCGTTGA
TCGACGTCCAACTCGGCACTCTGCGCAATTGGGAGCAAGGCCGCCGAGAACCGACAGGGCCGGCCAAAGCCCTGCTGAAA
GCCATTCACAATGACCCTGTCCATGTGTTGAATGCGTTAGCCGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J0EKU3

Antitoxin

Source ID Structure
AlphaFold DB A0A1J0EL37

References