Detailed information of TA system TA068241

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48809..49235
• Replicon: plasmid pKPGJ-2c
• Accession: NZ_CP017852
• Organism: Klebsiella variicola strain GJ2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BBD64_RS29280
• Protein ID: WP_001312861.1
• Coordinates: 48809..48967 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49011..49235 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD64_RS29240	44484..44867	-	384	Protein_46	conjugal transfer protein TrbJ	-
BBD64_RS29245	45061..45444	-	384	WP_001354030.1	relaxosome protein TraM	-
BBD64_RS29250	45767..46369	+	603	WP_077143832.1	transglycosylase SLT domain-containing protein	-
BBD64_RS29255	46666..47487	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BBD64_RS29260	47598..47894	-	297	WP_001272251.1	hypothetical protein	-
BBD64_RS29270	48120..48313	-	194	Protein_51	hypothetical protein	-
BBD64_RS29280	48809..48967	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49011..49235	-	225	NuclAT_0	-	Antitoxin
-	49011..49235	-	225	NuclAT_0	-	Antitoxin
-	49011..49235	-	225	NuclAT_0	-	Antitoxin
-	49011..49235	-	225	NuclAT_0	-	Antitoxin
BBD64_RS29285	49247..49966	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BBD64_RS29290	49963..50397	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BBD64_RS29295	50466..52489	-	2024	Protein_55	ParB/RepB/Spo0J family partition protein	-
BBD64_RS29300	52550..52783	-	234	WP_000006003.1	DUF905 family protein	-
BBD64_RS29305	52841..53368	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
BBD64_RS29315	53596..53789	-	194	Protein_58	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-215 / mph(A) / aph(3')-Ia	-	1..66447	66447
	-	flank	IS/Tn	-	-	43064..44332	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T68241 WP_001312861.1 NZ_CP017852:c48967-48809 [Klebsiella variicola]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T68241 NZ_CP017852:c48967-48809 [Klebsiella variicola]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT68241 NZ_CP017852:c49235-49011 [Klebsiella variicola]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin