Detailed information of TA system TA068240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 42277..43001
• Replicon: plasmid pKPGJ-2b
• Accession: NZ_CP017851
• Organism: Klebsiella variicola strain GJ2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: BBD64_RS28645
• Protein ID: WP_023292165.1
• Coordinates: 42277..42588 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBD64_RS28650
• Protein ID: WP_072122726.1
• Coordinates: 42585..43001 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD64_RS28625	38285..39187	-	903	WP_060598839.1	LysR family transcriptional regulator	-
BBD64_RS28630	39470..40609	+	1140	WP_072122725.1	PLP-dependent aspartate aminotransferase family protein	-
BBD64_RS28635	40630..41559	+	930	WP_158236771.1	DMT family transporter	-
BBD64_RS28640	41674..41975	+	302	Protein_48	DDE-type integrase/transposase/recombinase	-
BBD64_RS28645	42277..42588	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
BBD64_RS28650	42585..43001	+	417	WP_072122726.1	helix-turn-helix domain-containing protein	Antitoxin
BBD64_RS28655	43818..44198	+	381	WP_077138875.1	hypothetical protein	-
BBD64_RS28660	44264..44611	+	348	WP_077138876.1	hypothetical protein	-
BBD64_RS28665	44699..44929	+	231	WP_004197732.1	hypothetical protein	-
BBD64_RS28670	44976..45809	+	834	WP_077138877.1	type I restriction-modification system subunit M	-
BBD64_RS28675	46434..46964	+	531	WP_077138878.1	antirestriction protein	-
BBD64_RS28680	47002..47481	-	480	WP_077138879.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92232	92232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T68240 WP_023292165.1 NZ_CP017851:42277-42588 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T68240 NZ_CP017851:42277-42588 [Klebsiella variicola]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAGAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15468.55 Da
• Isoelectric Point: 4.4711

>AT68240 WP_072122726.1 NZ_CP017851:42585-43001 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDTWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT68240 NZ_CP017851:42585-43001 [Klebsiella variicola]
ATGATTTTTAGCGATGCCATTAAAGCTGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATACCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTCGAAAATGAGATAGGTAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3