Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5194256..5194902 Replicon chromosome
Accession NZ_CP017849
Organism Klebsiella variicola strain GJ2

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag BBD64_RS25805 Protein ID WP_008806991.1
Coordinates 5194555..5194902 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag BBD64_RS25800 Protein ID WP_008806992.1
Coordinates 5194256..5194555 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD64_RS25780 5190462..5191220 - 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -
BBD64_RS25785 5191270..5192100 - 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
BBD64_RS25790 5192151..5192480 - 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
BBD64_RS25795 5192685..5194193 + 1509 WP_032740278.1 glycerol-3-phosphate dehydrogenase -
BBD64_RS25800 5194256..5194555 - 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
BBD64_RS25805 5194555..5194902 - 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BBD64_RS25810 5195117..5197564 - 2448 WP_008806990.1 glycogen phosphorylase -
BBD64_RS25815 5197582..5199015 - 1434 WP_004202133.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T68237 WP_008806991.1 NZ_CP017849:c5194902-5194555 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T68237 NZ_CP017849:c5194902-5194555 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT68237 WP_008806992.1 NZ_CP017849:c5194555-5194256 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT68237 NZ_CP017849:c5194555-5194256 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCACTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References