Detailed information of TA system TA068192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 186817..187511
• Replicon: chromosome
• Accession: NZ_CP017844
• Organism: Escherichia coli strain FMU073332

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BLJ80_RS01090
• Protein ID: WP_001263493.1
• Coordinates: 186817..187215 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BLJ80_RS01095
• Protein ID: WP_000554757.1
• Coordinates: 187218..187511 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLJ80_RS01045	182376..182834	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BLJ80_RS01050	183095..184552	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BLJ80_RS01055	184609..185130	-	522	Protein_176	peptide chain release factor H	-
BLJ80_RS01060	185129..185320	-	192	Protein_177	RtcB family protein	-
BLJ80_RS28110	185370..186067	+	698	Protein_178	IS1 family transposase	-
BLJ80_RS01085	186355..186807	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BLJ80_RS01090	186817..187215	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BLJ80_RS01095	187218..187511	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BLJ80_RS01100	187563..188618	-	1056	WP_001226164.1	DNA polymerase IV	-
BLJ80_RS01105	188689..189612	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
BLJ80_RS01110	189615..190478	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
BLJ80_RS01115	190491..191210	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
BLJ80_RS01120	191230..191697	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	185564..186067	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T68192 WP_001263493.1 NZ_CP017844:c187215-186817 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T68192 NZ_CP017844:c187215-186817 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT68192 WP_000554757.1 NZ_CP017844:c187511-187218 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT68192 NZ_CP017844:c187511-187218 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6