Detailed information of TA system TA068190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1600971..1601635
• Replicon: chromosome
• Accession: NZ_CP017839
• Organism: Nocardia seriolae strain EM150506

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: NS506_RS07275
• Protein ID: WP_045435820.1
• Coordinates: 1601297..1601635 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0B8N7P4
• Locus tag: NS506_RS38450
• Protein ID: WP_071811585.1
• Coordinates: 1600971..1601300 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NS506_RS07250	1596184..1596948	-	765	WP_033085720.1	crotonase/enoyl-CoA hydratase family protein	-
NS506_RS07255	1597064..1597693	+	630	WP_071343557.1	TetR/AcrR family transcriptional regulator	-
NS506_RS07260	1597707..1599329	+	1623	WP_033085722.1	acyl-CoA dehydrogenase family protein	-
NS506_RS07265	1599519..1600901	+	1383	WP_071811584.1	DUF1731 domain-containing protein	-
NS506_RS38450	1600971..1601300	-	330	WP_071811585.1	helix-turn-helix domain-containing protein	Antitoxin
NS506_RS07275	1601297..1601635	-	339	WP_045435820.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NS506_RS07280	1601822..1602568	+	747	Protein_1459	cytochrome b	-
NS506_RS07285	1602565..1602804	+	240	WP_033085724.1	hypothetical protein	-
NS506_RS07290	1602805..1605276	-	2472	WP_036546081.1	DNA helicase PcrA	-
NS506_RS07295	1605597..1605902	+	306	WP_033085726.1	chorismate mutase	-
NS506_RS07300	1606104..1606550	-	447	WP_045435819.1	PaaI family thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (7-96)

Antitoxin

• HigA2-like, helix-turn-helix domain (41-98)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12892.56 Da
• Isoelectric Point: 4.8381

>T68190 WP_045435820.1 NZ_CP017839:c1601635-1601297 [Nocardia seriolae]
MKLCESDPETAALVEQALDRLAEVGPTLGRPLVDTLEHSRLRNLKELRPGSRGSSEIRMLFVFDPDREAIVLVAGDKAGR
WTRWYEEAIPLAEHRYADYRAEKDMRQQEEQP

Nucleotide

• Length: 339 bp

>T68190 NZ_CP017839:c1601635-1601297 [Nocardia seriolae]
ATGAAGCTGTGCGAGTCGGATCCAGAAACGGCGGCATTGGTCGAGCAGGCGTTGGACAGGCTTGCCGAGGTGGGCCCCAC
GCTCGGTCGTCCACTCGTCGATACGCTGGAGCACAGCCGACTACGCAATCTGAAGGAGCTGCGGCCCGGCTCCCGCGGTT
CTTCGGAGATCCGGATGTTGTTTGTCTTCGACCCGGACCGCGAAGCCATCGTTTTGGTCGCCGGCGACAAGGCCGGTCGG
TGGACTCGTTGGTACGAAGAGGCGATTCCGTTGGCTGAACATCGGTACGCGGACTATCGAGCCGAAAAAGACATGAGGCA
GCAGGAGGAGCAGCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11751.33 Da
• Isoelectric Point: 7.4681

>AT68190 WP_071811585.1 NZ_CP017839:c1601300-1600971 [Nocardia seriolae]
MSPARNWRDIKAEAHRLHPELADPAVRAEAAAHLDAYIAGYHLKELRKAAGKTQADVAAALRVSQSRVSQIENGDLGAME
LDTLRAYAEALGGRVEITLSVGQHTIKVA

Nucleotide

• Length: 330 bp

>AT68190 NZ_CP017839:c1601300-1600971 [Nocardia seriolae]
ATGAGCCCCGCGCGTAACTGGCGTGACATCAAGGCGGAAGCCCATCGCCTACATCCGGAACTGGCGGATCCCGCGGTACG
AGCCGAGGCGGCCGCACACCTCGATGCCTACATCGCGGGGTACCATCTGAAGGAACTCCGTAAGGCGGCGGGCAAGACCC
AAGCCGATGTTGCCGCCGCCCTTCGGGTTTCGCAGTCTCGTGTCTCGCAGATCGAGAACGGCGATCTCGGCGCAATGGAA
CTCGACACGCTCCGCGCCTACGCCGAAGCCCTTGGCGGTCGTGTCGAGATCACTCTCAGCGTCGGTCAGCATACGATCAA
GGTCGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0B8N7P4