Detailed information of TA system TA068182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2494202..2494859
• Replicon: chromosome
• Accession: NZ_CP017810
• Organism: Thalassolituus oleivorans strain K188

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1L6KEJ0
• Locus tag: CN03_RS11525
• Protein ID: WP_075273878.1
• Coordinates: 2494202..2494555 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CN03_RS11530
• Protein ID: WP_025265809.1
• Coordinates: 2494557..2494859 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN03_RS11510	2489896..2490357	+	462	WP_075273875.1	DNA polymerase III subunit chi	-
CN03_RS11515	2490357..2490920	+	564	WP_075273876.1	hypothetical protein	-
CN03_RS11520	2491065..2494007	+	2943	WP_075273877.1	valine--tRNA ligase	-
CN03_RS11525	2494202..2494555	+	354	WP_075273878.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CN03_RS11530	2494557..2494859	+	303	WP_025265809.1	XRE family transcriptional regulator	Antitoxin
CN03_RS11540	2495316..2495735	-	420	WP_075273880.1	hypothetical protein	-
CN03_RS11545	2495974..2496678	-	705	WP_075273882.1	hypothetical protein	-
CN03_RS11550	2497123..2498037	-	915	WP_075273884.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13499.48 Da
• Isoelectric Point: 7.1666

>T68182 WP_075273878.1 NZ_CP017810:2494202-2494555 [Thalassolituus oleivorans]
MWHIETTDTFDTWFDALDDTDRANVLASMIALRERGPMLPRPYADTVKGSSFSNMKELRVQSKGDPILAFFAFDPKRQGI
LLCAGNKTNDEKRFYEVMIPIADREFTLHLKQLKQSK

Nucleotide

• Length: 354 bp

>T68182 NZ_CP017810:2494202-2494555 [Thalassolituus oleivorans]
ATGTGGCACATAGAAACAACAGATACCTTTGATACTTGGTTCGATGCTCTCGATGATACCGACCGGGCCAATGTACTGGC
CTCAATGATAGCGCTACGAGAACGTGGCCCTATGCTCCCAAGGCCATACGCGGACACGGTAAAAGGATCGTCATTCAGCA
ACATGAAAGAGCTAAGAGTTCAAAGCAAAGGCGACCCAATACTAGCCTTCTTTGCCTTTGATCCAAAACGACAAGGCATC
CTTCTATGTGCAGGCAACAAAACCAATGATGAAAAGCGGTTTTATGAGGTAATGATCCCCATCGCAGATCGTGAATTCAC
CTTGCACTTAAAACAGTTAAAGCAGAGTAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11060.79 Da
• Isoelectric Point: 8.5277

>AT68182 WP_025265809.1 NZ_CP017810:2494557-2494859 [Thalassolituus oleivorans]
MARTLDDILANEKPAVVAKAQKAATEILLNIRLAELRERMNLTQGDIAETLGVKQPTVSDMEKPGRDLKLSSLKRYVEAS
GGKVRIDIELPDGTHYGFRI

Nucleotide

• Length: 303 bp

>AT68182 NZ_CP017810:2494557-2494859 [Thalassolituus oleivorans]
ATGGCTAGAACACTCGACGACATCCTTGCTAACGAAAAACCAGCCGTTGTTGCAAAAGCCCAAAAAGCAGCAACAGAAAT
ATTACTCAATATCCGCTTGGCAGAACTCCGTGAACGCATGAATCTAACTCAGGGGGATATAGCCGAAACTCTAGGAGTGA
AACAGCCGACAGTATCGGACATGGAAAAACCCGGACGTGATTTAAAGCTATCGTCTTTGAAGCGTTATGTCGAAGCATCT
GGTGGAAAGGTACGAATTGATATCGAACTGCCGGACGGGACACACTATGGGTTTAGGATATAG

Structures

Toxin

• AlphaFold DB: A0A1L6KEJ0