Detailed information of TA system TA068075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1219001..1219565
• Replicon: chromosome
• Accession: NZ_CP017716
• Organism: Lacticaseibacillus paracasei strain TK1501

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: BKQ19_RS06325
• Protein ID: WP_094515984.1
• Coordinates: 1219263..1219565 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: K6SLT0
• Locus tag: BKQ19_RS06320
• Protein ID: WP_003570051.1
• Coordinates: 1219001..1219270 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKQ19_RS06290	1214006..1214893	-	888	WP_094515983.1	ABC transporter ATP-binding protein	-
BKQ19_RS06295	1215090..1216316	-	1227	WP_003570043.1	hypothetical protein	-
BKQ19_RS06300	1216514..1216918	+	405	WP_003570045.1	MerR family transcriptional regulator	-
BKQ19_RS06305	1216911..1217237	+	327	WP_003584272.1	YrdB family protein	-
BKQ19_RS06310	1217300..1217929	+	630	WP_003570047.1	DUF2399 domain-containing protein	-
BKQ19_RS06315	1217974..1218627	+	654	WP_003570049.1	GNAT family N-acetyltransferase	-
BKQ19_RS06320	1219001..1219270	+	270	WP_003570051.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BKQ19_RS06325	1219263..1219565	+	303	WP_094515984.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BKQ19_RS06330	1220025..1221290	-	1266	WP_157698601.1	IS1380 family transposase	-
BKQ19_RS15215	1221531..1221674	+	144	WP_162259784.1	hypothetical protein	-
BKQ19_RS06335	1221792..1222484	+	693	WP_003570055.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-97)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11510.33 Da
• Isoelectric Point: 9.8881

>T68075 WP_094515984.1 NZ_CP017716:1219263-1219565 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAEILSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Nucleotide

• Length: 303 bp

>T68075 NZ_CP017716:1219263-1219565 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAAATATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9982.68 Da
• Isoelectric Point: 7.2026

>AT68075 WP_003570051.1 NZ_CP017716:1219001-1219270 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT68075 NZ_CP017716:1219001-1219270 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E2BRK6