Detailed information of TA system TA068073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1666522..1667093
• Replicon: chromosome
• Accession: NZ_CP017715
• Organism: Marinobacter salinus strain Hb8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1D9GKH0
• Locus tag: BKP64_RS07635
• Protein ID: WP_031211838.1
• Coordinates: 1666815..1667093 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BKP64_RS07630
• Protein ID: WP_023012140.1
• Coordinates: 1666522..1666815 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKP64_RS07595	1661548..1661820	-	273	WP_070968099.1	ribbon-helix-helix protein, CopG family	-
BKP64_RS07600	1661965..1662228	-	264	WP_070968102.1	Txe/YoeB family addiction module toxin	-
BKP64_RS07605	1662225..1662467	-	243	WP_058092928.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BKP64_RS07610	1662642..1663400	-	759	WP_070968105.1	transglutaminase family protein	-
BKP64_RS07615	1663475..1664170	-	696	WP_157755409.1	helix-turn-helix domain-containing protein	-
BKP64_RS07620	1664522..1665430	-	909	WP_198402660.1	ZIP family metal transporter	-
BKP64_RS07625	1665402..1666387	-	986	Protein_1499	transposase	-
BKP64_RS07630	1666522..1666815	-	294	WP_023012140.1	HigA family addiction module antidote protein	Antitoxin
BKP64_RS07635	1666815..1667093	-	279	WP_031211838.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKP64_RS07640	1667325..1668896	-	1572	WP_070968112.1	transposase	-
BKP64_RS07645	1669044..1669607	-	564	WP_070968115.1	hypothetical protein	-
BKP64_RS07650	1669724..1671295	-	1572	WP_070968112.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1656095..1683320	27225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10743.22 Da
• Isoelectric Point: 10.0257

>T68073 WP_031211838.1 NZ_CP017715:c1667093-1666815 [Marinobacter salinus]
MIKSFRHKGLKRFYSTGHTSGIQPDHAKKLRMQLAALDTATSVEDMDIPGFRLHPLKGKDKGRWSIRVNGNWRMTFEFQD
GNAYILDYEDYH

Nucleotide

• Length: 279 bp

>T68073 NZ_CP017715:c1667093-1666815 [Marinobacter salinus]
ATGATTAAATCATTCCGTCACAAAGGACTGAAGCGGTTTTACTCGACCGGCCACACGTCTGGCATACAACCCGACCATGC
CAAGAAGCTGCGTATGCAGCTGGCTGCTCTGGATACGGCGACTTCAGTGGAAGACATGGATATCCCTGGTTTTCGGCTTC
ATCCATTGAAGGGCAAAGACAAAGGCCGGTGGTCGATTAGGGTTAACGGAAATTGGCGCATGACGTTCGAATTCCAGGAC
GGCAACGCCTACATTCTTGATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10770.33 Da
• Isoelectric Point: 6.2137

>AT68073 WP_023012140.1 NZ_CP017715:c1666815-1666522 [Marinobacter salinus]
MTMHNPPHPGEFIREVYLEPFGISSRQLASSLGVSPSTLSRLLKGDSGISPEMSLRLSKVLGRTPESWLAMQDMYDLWVA
RGTVNLDGIHRLDFEAA

Nucleotide

• Length: 294 bp

>AT68073 NZ_CP017715:c1666815-1666522 [Marinobacter salinus]
ATGACTATGCATAATCCGCCGCACCCTGGTGAATTCATTCGCGAGGTGTACCTGGAGCCTTTCGGTATCAGCTCTCGTCA
GCTTGCTTCCAGTCTGGGCGTTTCACCTTCTACTTTGTCTCGGCTGCTCAAAGGAGATAGTGGCATTAGCCCTGAAATGT
CTTTGCGGCTGTCCAAGGTTCTCGGGCGCACTCCTGAGAGCTGGTTGGCGATGCAAGATATGTATGACCTATGGGTGGCT
CGCGGCACAGTCAATCTGGATGGTATTCATCGCCTGGACTTCGAAGCAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1D9GKH0