Detailed information of TA system TA068060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3813758..3814329
• Replicon: chromosome
• Accession: NZ_CP017689
• Organism: Thalassotalea crassostreae strain LPB0090

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Y0DPS6
• Locus tag: LT090_RS16520
• Protein ID: WP_068547700.1
• Coordinates: 3814051..3814329 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LT090_RS16515
• Protein ID: WP_068547701.1
• Coordinates: 3813758..3814051 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT090_RS16465	3808800..3809207	-	408	WP_068547711.1	GFA family protein	-
LT090_RS16470	3809409..3810059	+	651	WP_068547710.1	histidine kinase	-
LT090_RS16475	3810078..3810527	-	450	WP_068547709.1	nuclear transport factor 2 family protein	-
LT090_RS16480	3810551..3811198	-	648	WP_068547708.1	DUF4386 family protein	-
LT090_RS16485	3811318..3811563	-	246	WP_068547707.1	Rho-binding antiterminator	-
LT090_RS16490	3811627..3811980	-	354	WP_068547706.1	DUF2200 domain-containing protein	-
LT090_RS16495	3812029..3812439	-	411	WP_068547705.1	hypothetical protein	-
LT090_RS16500	3812492..3812680	-	189	WP_068547704.1	hypothetical protein	-
LT090_RS16505	3812838..3813119	-	282	WP_068547703.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LT090_RS16510	3813306..3813650	-	345	WP_068547702.1	hypothetical protein	-
LT090_RS16515	3813758..3814051	-	294	WP_068547701.1	HigA family addiction module antidote protein	Antitoxin
LT090_RS16520	3814051..3814329	-	279	WP_068547700.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT090_RS16525	3814542..3815585	+	1044	WP_068547699.1	IS110 family transposase	-
LT090_RS17040	3815829..3815972	+	144	WP_157726661.1	hypothetical protein	-
LT090_RS16530	3816047..3816493	-	447	WP_157726666.1	hypothetical protein	-
LT090_RS16535	3816590..3817219	-	630	WP_068547896.1	PEP-CTERM sorting domain-containing protein	-
LT090_RS16540	3817513..3817758	-	246	WP_068547895.1	hypothetical protein	-
LT090_RS16545	3817882..3818178	-	297	WP_068547894.1	HigA family addiction module antidote protein	-
LT090_RS16550	3818178..3818456	-	279	WP_068547893.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3814542..3815585	1043
	-	inside	Integron	-	-	3813664..3829303	15639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.21 Da
• Isoelectric Point: 9.7581

>T68060 WP_068547700.1 NZ_CP017689:c3814329-3814051 [Thalassotalea crassostreae]
MIKSFKHKGLKKYFQTGSVAGIQAKHQRKLRMQLTAIDTAQEIDDINLPGFKLHPLKGDRDGIWSITVNGNWRITFEFID
GNAYILNYEDYH

Nucleotide

• Length: 279 bp

>T68060 NZ_CP017689:c3814329-3814051 [Thalassotalea crassostreae]
ATGATTAAATCTTTTAAGCACAAAGGCCTCAAAAAGTATTTCCAAACTGGAAGTGTTGCTGGAATACAGGCTAAGCATCA
ACGTAAATTACGGATGCAACTTACCGCAATAGATACCGCGCAAGAAATTGATGATATCAATTTACCCGGTTTTAAATTGC
ACCCTCTTAAAGGGGATCGTGACGGGATTTGGTCTATTACTGTAAATGGCAACTGGCGCATTACTTTTGAGTTTATCGAT
GGTAATGCATACATTCTCAACTATGAGGACTATCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10918.46 Da
• Isoelectric Point: 7.3556

>AT68060 WP_068547701.1 NZ_CP017689:c3814051-3813758 [Thalassotalea crassostreae]
MTMHNPPHPGEFIYDVYLEPAGFSCRYLAKQLDVASSTLNRVLKGQSAISPEMALRLSKAIGRTPESWLAMQDNYDLWHA
KQQINLSNVHSVNFTVA

Nucleotide

• Length: 294 bp

>AT68060 NZ_CP017689:c3814051-3813758 [Thalassotalea crassostreae]
ATGACTATGCATAATCCGCCGCATCCTGGAGAGTTTATCTATGACGTTTACCTAGAGCCTGCTGGCTTTAGCTGTCGTTA
TTTAGCTAAGCAATTAGATGTAGCATCATCTACTTTAAACCGTGTTCTTAAAGGTCAAAGTGCTATCTCTCCTGAAATGG
CACTTCGCTTATCTAAAGCAATTGGCAGAACGCCAGAAAGTTGGCTAGCAATGCAAGATAACTATGATCTTTGGCATGCT
AAACAACAAATTAATTTAAGTAATGTTCACTCAGTAAACTTTACGGTAGCATAA

Structures

Toxin

• AlphaFold DB: A0A7Y0DPS6