Detailed information of TA system TA068041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2094040..2094463
• Replicon: chromosome
• Accession: NZ_CP017684
• Organism: Staphylococcus aureus strain CFSAN007847

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: AB520_RS10735
• Protein ID: WP_001065091.1
• Coordinates: 2094212..2094463 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: AB520_RS10730
• Protein ID: WP_000028422.1
• Coordinates: 2094040..2094222 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB520_RS10685	2089426..2091087	-	1662	WP_000625088.1	terminase large subunit	-
AB520_RS10690	2091084..2091428	-	345	WP_000402904.1	hypothetical protein	-
AB520_RS10695	2091558..2091857	-	300	WP_000988336.1	HNH endonuclease	-
AB520_RS10700	2092089..2092505	-	417	WP_000590122.1	hypothetical protein	-
AB520_RS10705	2092533..2092733	-	201	WP_000265043.1	DUF1514 family protein	-
AB520_RS10710	2092733..2092882	-	150	WP_000595265.1	transcriptional activator RinB	-
AB520_RS10715	2092879..2093265	-	387	WP_000592207.1	hypothetical protein	-
AB520_RS10720	2093262..2093468	-	207	WP_000195803.1	DUF1381 domain-containing protein	-
AB520_RS10725	2093505..2094047	-	543	WP_099119667.1	dUTP pyrophosphatase	-
AB520_RS10730	2094040..2094222	-	183	WP_000028422.1	hypothetical protein	Antitoxin
AB520_RS10735	2094212..2094463	-	252	WP_001065091.1	DUF1024 family protein	Toxin
AB520_RS14895	2094456..2094623	-	168	WP_001624242.1	hypothetical protein	-
AB520_RS10740	2094635..2094877	-	243	WP_000131366.1	hypothetical protein	-
AB520_RS10745	2094881..2095249	-	369	WP_099119668.1	hypothetical protein	-
AB520_RS10750	2095262..2095571	-	310	Protein_1996	RusA family crossover junction endodeoxyribonuclease	-
AB520_RS10755	2095580..2095798	-	219	WP_000338531.1	hypothetical protein	-
AB520_RS10760	2095805..2096678	-	874	Protein_1998	DnaD domain protein	-
AB520_RS10765	2096708..2097178	-	471	WP_000934764.1	single-stranded DNA-binding protein	-
AB520_RS10770	2097179..2097796	-	618	WP_071621397.1	MBL fold metallo-hydrolase	-
AB520_RS10775	2097877..2098797	-	921	WP_000138472.1	recombinase RecT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	map / hlb / scn / chp / sak / sea / hlb / tsst-1 / groEL	2060598..2134325	73727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T68041 WP_001065091.1 NZ_CP017684:c2094463-2094212 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T68041 NZ_CP017684:c2094463-2094212 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT68041 WP_000028422.1 NZ_CP017684:c2094222-2094040 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT68041 NZ_CP017684:c2094222-2094040 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8