Detailed information of TA system TA068017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 862231..862645
• Replicon: chromosome
• Accession: NZ_CP017682
• Organism: Staphylococcus aureus strain CFSAN007850

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AB526_RS04360
• Protein ID: WP_025174939.1
• Coordinates: 862231..862482 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AB526_RS14715
• Protein ID: WP_000028424.1
• Coordinates: 862472..862645 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB526_RS04320	857895..858815	+	921	WP_000138475.1	recombinase RecT	-
AB526_RS04325	858896..859513	+	618	WP_071621397.1	MBL fold metallo-hydrolase	-
AB526_RS04330	859514..859984	+	471	WP_000934770.1	single-stranded DNA-binding protein	-
AB526_RS04335	860014..860898	+	885	WP_000148301.1	DnaD domain protein	-
AB526_RS04340	860905..861123	+	219	WP_000338528.1	hypothetical protein	-
AB526_RS04345	861132..861587	+	456	WP_000401965.1	RusA family crossover junction endodeoxyribonuclease	-
AB526_RS04350	861600..861970	+	371	Protein_807	hypothetical protein	-
AB526_RS04355	861974..862216	+	243	WP_000131381.1	phi PVL orf 51-like protein	-
AB526_RS04360	862231..862482	+	252	WP_025174939.1	DUF1024 family protein	Toxin
AB526_RS14715	862472..862645	+	174	WP_000028424.1	hypothetical protein	Antitoxin
AB526_RS04365	862646..862927	+	282	WP_000454994.1	hypothetical protein	-
AB526_RS14720	862928..863089	+	162	WP_000889683.1	hypothetical protein	-
AB526_RS04370	863104..863637	+	534	WP_025920701.1	dUTP diphosphatase	-
AB526_RS04375	863674..863880	+	207	WP_000195826.1	DUF1381 domain-containing protein	-
AB526_RS04380	863877..864263	+	387	WP_000592207.1	hypothetical protein	-
AB526_RS04385	864260..864433	+	174	WP_031925651.1	transcriptional activator RinB	-
AB526_RS04390	864434..864580	+	147	WP_000990003.1	hypothetical protein	-
AB526_RS04395	864595..865014	+	420	WP_094637849.1	transcriptional regulator	-
AB526_RS04400	865201..865641	+	441	WP_001003271.1	terminase small subunit	-
AB526_RS04405	865628..866905	+	1278	WP_000169945.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	848287..898109	49822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9361.22 Da
• Isoelectric Point: 3.8464

>T68017 WP_025174939.1 NZ_CP017682:862231-862482 [Staphylococcus aureus]
MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILDGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T68017 NZ_CP017682:862231-862482 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT68017 WP_000028424.1 NZ_CP017682:862472-862645 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT68017 NZ_CP017682:862472-862645 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA