Detailed information of TA system TA067958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4908270..4908964
• Replicon: chromosome
• Accession: NZ_CP017669
• Organism: Escherichia coli strain PA20

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: AU473_RS25435
• Protein ID: WP_001263495.1
• Coordinates: 4908270..4908668 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AU473_RS25440
• Protein ID: WP_000554757.1
• Coordinates: 4908671..4908964 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4903857..4903937	-	81	NuclAT_12	-	-
-	4903857..4903937	-	81	NuclAT_12	-	-
-	4903857..4903937	-	81	NuclAT_12	-	-
-	4903857..4903937	-	81	NuclAT_12	-	-
AU473_RS25410	4904533..4904991	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AU473_RS25415	4905252..4906709	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AU473_RS25420	4906766..4907287	-	522	Protein_4822	peptide chain release factor H	-
AU473_RS25425	4907286..4907489	-	204	Protein_4823	RNA ligase RtcB family protein	-
AU473_RS25430	4907808..4908260	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
AU473_RS25435	4908270..4908668	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AU473_RS25440	4908671..4908964	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AU473_RS25445	4909016..4910071	-	1056	WP_001226188.1	DNA polymerase IV	-
AU473_RS25450	4910142..4910927	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AU473_RS25455	4910899..4912611	+	1713	Protein_4829	flagellar biosynthesis protein FlhA	-
AU473_RS32235	4912756..4913253	-	498	Protein_4830	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4871429..4914202	42773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T67958 WP_001263495.1 NZ_CP017669:c4908668-4908270 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T67958 NZ_CP017669:c4908668-4908270 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT67958 WP_000554757.1 NZ_CP017669:c4908964-4908671 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT67958 NZ_CP017669:c4908964-4908671 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6