Detailed information of TA system TA067888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1473596..1474172
• Replicon: chromosome
• Accession: NZ_CP017639
• Organism: Corynebacterium sp. NML98-0116

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A1J5VZN4
• Locus tag: BJP05_RS06640
• Protein ID: WP_070362732.1
• Coordinates: 1473891..1474172 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BJP05_RS06635
• Protein ID: WP_071573317.1
• Coordinates: 1473596..1473904 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJP05_RS06615	1469050..1469409	+	360	WP_070362728.1	metalloregulator ArsR/SmtB family transcription factor	-
BJP05_RS06620	1469406..1471298	+	1893	WP_070362729.1	cadmium-translocating P-type ATPase	-
BJP05_RS06625	1471377..1472357	-	981	WP_070362730.1	NAD(P)/FAD-dependent oxidoreductase	-
BJP05_RS10990	1472577..1473027	+	451	Protein_1297	putative glycolipid-binding domain-containing protein	-
BJP05_RS06630	1473098..1473541	+	444	WP_070362731.1	aminoacyl-tRNA hydrolase	-
BJP05_RS06635	1473596..1473904	-	309	WP_071573317.1	HigA family addiction module antidote protein	Antitoxin
BJP05_RS06640	1473891..1474172	-	282	WP_070362732.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJP05_RS06645	1474666..1475646	+	981	Protein_1301	IS21 family transposase	-
BJP05_RS06650	1475765..1476025	+	261	Protein_1302	transposase	-
BJP05_RS06655	1476055..1477788	-	1734	WP_070362735.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1475765..1476103	338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.45 Da
• Isoelectric Point: 9.8469

>T67888 WP_070362732.1 NZ_CP017639:c1474172-1473891 [Corynebacterium sp. NML98-0116]
VIQSFADKDTERLWNRERVRSIDSRIHSVALRKLRQLGYAQTVDELRIPPGNRLEALKGDRRGQFSIRINDQWRICFRWS
AAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T67888 NZ_CP017639:c1474172-1473891 [Corynebacterium sp. NML98-0116]
GTGATCCAGTCGTTCGCTGACAAAGACACTGAGCGTTTGTGGAATCGAGAGCGGGTTCGCTCGATCGATTCCCGCATTCA
CTCAGTAGCGCTGCGCAAGCTTCGCCAGCTTGGGTACGCGCAGACTGTTGATGAGTTACGGATTCCCCCGGGGAACCGGC
TTGAGGCGTTGAAAGGTGATCGGCGGGGTCAGTTCAGCATTCGAATCAACGACCAGTGGCGGATCTGTTTTCGGTGGTCT
GCCGCGGGGCCAGAGGAGGTTGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11403.29 Da
• Isoelectric Point: 6.4817

>AT67888 WP_071573317.1 NZ_CP017639:c1473904-1473596 [Corynebacterium sp. NML98-0116]
MTTTDKLPPIHPGEILMEDFLKEMGITQHKLAVSIGVPPRRINEIVHGKRAVTADTALRLAKFFGMSPQFWLGLQTQYDL
DVAEDKILAEIERIQPVHAVSA

Nucleotide

• Length: 309 bp

>AT67888 NZ_CP017639:c1473904-1473596 [Corynebacterium sp. NML98-0116]
TTGACTACCACTGACAAGCTCCCTCCGATTCACCCCGGTGAGATCCTCATGGAGGACTTTCTCAAGGAAATGGGGATCAC
CCAGCACAAGCTCGCAGTCTCCATCGGCGTTCCGCCCCGCCGGATCAACGAAATTGTTCACGGTAAGCGCGCTGTAACCG
CGGACACTGCTCTTCGCTTGGCTAAGTTCTTCGGAATGAGCCCCCAGTTCTGGCTCGGGTTACAGACCCAGTACGACCTG
GATGTGGCAGAAGACAAAATCCTCGCCGAGATCGAGAGGATTCAGCCGGTTCACGCTGTCTCGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A1J5VZN4