Detailed information of TA system TA067857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 674512..675145
• Replicon: chromosome
• Accession: NZ_CP017635
• Organism: Vibrio vulnificus Env1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BJD94_RS03470
• Protein ID: WP_103154571.1
• Coordinates: 674512..674844 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2K8XYD2
• Locus tag: BJD94_RS03475
• Protein ID: WP_071979346.1
• Coordinates: 674831..675145 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD94_RS03400	669705..669797	+	93	WP_005448104.1	DUF3265 domain-containing protein	-
BJD94_RS03410	670164..670292	+	129	WP_103155413.1	DUF3265 domain-containing protein	-
BJD94_RS03415	670641..670763	+	123	WP_079451831.1	DUF3265 domain-containing protein	-
BJD94_RS03420	670801..671109	+	309	WP_017428770.1	hypothetical protein	-
BJD94_RS03425	671127..671228	+	102	WP_072605994.1	DUF3265 domain-containing protein	-
BJD94_RS03430	671268..671687	+	420	WP_038940638.1	RDD family protein	-
BJD94_RS03435	671829..672179	+	351	WP_103154564.1	hypothetical protein	-
BJD94_RS03440	672324..672803	+	480	WP_039542963.1	GNAT family N-acetyltransferase	-
BJD94_RS03450	673207..673359	+	153	WP_193783781.1	DUF3265 domain-containing protein	-
BJD94_RS03455	673362..673820	+	459	WP_050905150.1	GNAT family N-acetyltransferase	-
BJD94_RS03460	673846..673938	+	93	WP_012841684.1	DUF3265 domain-containing protein	-
BJD94_RS03465	674299..674475	+	177	WP_103154572.1	DUF3265 domain-containing protein	-
BJD94_RS03470	674512..674844	+	333	WP_103154571.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJD94_RS03475	674831..675145	+	315	WP_071979346.1	DNA-binding transcriptional regulator	Antitoxin
BJD94_RS23455	675614..675733	+	120	Protein_666	DUF3265 domain-containing protein	-
BJD94_RS03485	675765..676214	+	450	WP_044128179.1	DUF2513 domain-containing protein	-
BJD94_RS03490	676211..676321	+	111	WP_081024888.1	DUF3265 domain-containing protein	-
BJD94_RS03495	676644..676808	+	165	WP_193783784.1	DUF3265 domain-containing protein	-
BJD94_RS03500	677151..677279	+	129	WP_103154584.1	DUF3265 domain-containing protein	-
BJD94_RS03510	677622..677744	+	123	WP_103154583.1	DUF3265 domain-containing protein	-
BJD94_RS03515	677769..678809	+	1041	WP_103154582.1	GTP pyrophosphokinase	-
BJD94_RS03520	678824..678916	+	93	WP_193783783.1	DUF3265 domain-containing protein	-
BJD94_RS03530	679277..679399	+	123	WP_103155501.1	DUF3265 domain-containing protein	-
BJD94_RS03535	679416..679937	+	522	WP_017420670.1	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flp1	361742..900507	538765
	-	inside	Integron	-	-	611180..687587	76407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12902.81 Da
• Isoelectric Point: 10.0960

>T67857 WP_103154571.1 NZ_CP017635:674512-674844 [Vibrio vulnificus Env1]
MKSVFVESTIFEKYRNDYLSDEEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMADLIADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T67857 NZ_CP017635:674512-674844 [Vibrio vulnificus Env1]
ATGAAAAGTGTATTTGTTGAATCAACCATATTTGAAAAGTACCGAAATGATTACCTCAGTGACGAGGAGTTTCGATTGTT
CCAAGCTGAACTTATGTCGAACCCGAAGCAAGGTGACGTAATTCAAGGTACTGGCGGTTTGCGGAAGATTCGAGTAGCAA
GTAAAGGTAAGGGAAAGCGTGGTGGTTCACGTGTTATCTATTATTTCCTCGATGAAAAGCGTCGCTTCTATTTGCTGACT
ATTTACGGCAAAAATGAAATGGCAGACTTAATCGCAGATCAAAAGAAACAGTTAAAAGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11718.16 Da
• Isoelectric Point: 5.9765

>AT67857 WP_071979346.1 NZ_CP017635:674831-675145 [Vibrio vulnificus Env1]
MSNRDLFAELNSALVEAKEHSEGKVTLKTHQVNDISELDISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT67857 NZ_CP017635:674831-675145 [Vibrio vulnificus Env1]
ATGAGCAATCGTGATCTATTTGCAGAGCTAAATTCTGCACTTGTTGAAGCTAAGGAGCATTCAGAGGGCAAAGTGACTCT
GAAAACTCATCAAGTTAATGACATCAGTGAGCTAGATATCTCACCTGATGAAATTGTTAGTATTCGTGAGCAGTTTAATA
TGTCTCGTGGTGTATTTGCTCGTTTACTGCATACATCATCACGTACATTGGAAAACTGGGAGCAGGGGCGGAGCGCACCA
AATGGGCAAGCTGTTACTCTTCTAAAGCTGGTACAGCGCCACCCAGAAACTCTGTCGCACATCGCAGAGCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8XYD2