Detailed information of TA system TA067847

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64586..64839
• Replicon: plasmid pSLK172-2
• Accession: NZ_CP017633
• Organism: Escherichia coli strain SLK172

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: BJJ90_RS31360
• Protein ID: WP_001312851.1
• Coordinates: 64586..64735 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64780..64839 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ90_RS31310	59945..60360	-	416	Protein_69	IS1 family transposase	-
BJJ90_RS31320	60609..61010	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
BJJ90_RS33585	60943..61200	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BJJ90_RS31330	61293..61946	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BJJ90_RS33750	62044..62184	-	141	WP_001333237.1	hypothetical protein	-
BJJ90_RS31340	62885..63742	-	858	WP_075362754.1	incFII family plasmid replication initiator RepA	-
BJJ90_RS31345	63735..63809	-	75	WP_001375168.1	RepA leader peptide Tap	-
BJJ90_RS31355	64054..64302	-	249	WP_000083837.1	replication regulatory protein RepA	-
BJJ90_RS31360	64586..64735	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	64780..64839	+	60	NuclAT_1	-	Antitoxin
-	64780..64839	+	60	NuclAT_1	-	Antitoxin
-	64780..64839	+	60	NuclAT_1	-	Antitoxin
-	64780..64839	+	60	NuclAT_1	-	Antitoxin
BJJ90_RS31365	65040..65270	-	231	WP_001736714.1	hypothetical protein	-
BJJ90_RS31370	65434..66033	-	600	WP_032083981.1	hypothetical protein	-
BJJ90_RS31375	66419..66619	-	201	WP_015059022.1	hypothetical protein	-
BJJ90_RS31380	66751..67311	-	561	WP_000139328.1	fertility inhibition protein FinO	-
BJJ90_RS31385	67366..68112	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..120528	120528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T67847 WP_001312851.1 NZ_CP017633:c64735-64586 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T67847 NZ_CP017633:c64735-64586 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT67847 NZ_CP017633:64780-64839 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin