Detailed information of TA system TA067837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4163698..4164392
• Replicon: chromosome
• Accession: NZ_CP017631
• Organism: Escherichia coli strain SLK172

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BJJ90_RS22675
• Protein ID: WP_001263493.1
• Coordinates: 4163698..4164096 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BJJ90_RS22680
• Protein ID: WP_000554757.1
• Coordinates: 4164099..4164392 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ90_RS22645	4158698..4159942	-	1245	WP_000189541.1	esterase FrsA	-
-	4159358..4159438	-	81	NuclAT_9	-	-
-	4159358..4159438	-	81	NuclAT_9	-	-
-	4159358..4159438	-	81	NuclAT_9	-	-
-	4159358..4159438	-	81	NuclAT_9	-	-
BJJ90_RS22650	4160034..4160492	-	459	WP_001291994.1	xanthine phosphoribosyltransferase	-
BJJ90_RS22655	4160753..4162210	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BJJ90_RS22660	4162267..4162788	-	522	Protein_4020	peptide chain release factor H	-
BJJ90_RS22665	4162787..4162990	-	204	Protein_4021	RNA ligase RtcB family protein	-
BJJ90_RS22670	4163236..4163688	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BJJ90_RS22675	4163698..4164096	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BJJ90_RS22680	4164099..4164392	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BJJ90_RS22685	4164444..4165499	-	1056	WP_001226164.1	DNA polymerase IV	-
BJJ90_RS22690	4165570..4166355	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
BJJ90_RS22695	4166327..4168039	+	1713	Protein_4027	flagellar biosynthesis protein FlhA	-
BJJ90_RS22705	4168263..4168759	-	497	Protein_4028	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4110153..4164392	54239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T67837 WP_001263493.1 NZ_CP017631:c4164096-4163698 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T67837 NZ_CP017631:c4164096-4163698 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT67837 WP_000554757.1 NZ_CP017631:c4164392-4164099 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT67837 NZ_CP017631:c4164392-4164099 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6