Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1947233..1947735 Replicon chromosome
Accession NZ_CP017631
Organism Escherichia coli strain SLK172

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BJJ90_RS10215 Protein ID WP_000767829.1
Coordinates 1947481..1947735 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BJJ90_RS10210 Protein ID WP_001259255.1
Coordinates 1947233..1947484 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJJ90_RS10180 1942411..1943478 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BJJ90_RS10185 1943478..1944548 - 1071 WP_075362563.1 histidinol-phosphate transaminase -
BJJ90_RS10190 1944545..1945849 - 1305 WP_021562654.1 histidinol dehydrogenase -
BJJ90_RS10195 1945855..1946754 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BJJ90_RS10205 1946900..1946950 - 51 WP_001364200.1 his operon leader peptide -
BJJ90_RS10210 1947233..1947484 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BJJ90_RS10215 1947481..1947735 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BJJ90_RS10220 1947818..1948642 + 825 WP_000754758.1 SDR family oxidoreductase -
BJJ90_RS10225 1948688..1949617 + 930 WP_000803351.1 LysR family transcriptional regulator -
BJJ90_RS32695 1949832..1949894 + 63 WP_010723108.1 membrane protein YoeI -
BJJ90_RS10230 1949884..1951242 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BJJ90_RS10235 1951421..1952479 + 1059 WP_072656680.1 transport protein YeeE -
BJJ90_RS10240 1952493..1952720 + 228 WP_000985278.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T67817 WP_000767829.1 NZ_CP017631:1947481-1947735 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T67817 NZ_CP017631:1947481-1947735 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT67817 WP_001259255.1 NZ_CP017631:1947233-1947484 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT67817 NZ_CP017631:1947233-1947484 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References