67725

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 3001515..3002163 Replicon chromosome
Accession NZ_CP017597
Organism Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag BJM03_RS14665 Protein ID WP_003899414.1
Coordinates 3001515..3001838 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag BJM03_RS14670 Protein ID WP_003899415.1
Coordinates 3001918..3002163 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM03_RS14635 2996838..2997836 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
BJM03_RS14640 2997850..2998314 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
BJM03_RS14645 2998302..2998553 + 252 WP_003908028.1 hypothetical protein -
BJM03_RS14650 2998724..3000163 - 1440 WP_003901443.1 phage major capsid protein -
BJM03_RS14655 3000171..3000704 - 534 WP_003899412.1 HK97 family phage prohead protease -
BJM03_RS14660 3000857..3001348 - 492 WP_003900541.1 phage terminase small subunit P27 family -
BJM03_RS14665 3001515..3001838 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
BJM03_RS14670 3001918..3002163 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
BJM03_RS14675 3002160..3003587 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
BJM03_RS14680 3003589..3003981 - 393 WP_003899417.1 DUF2742 domain-containing protein -
BJM03_RS14685 3003978..3004238 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
BJM03_RS14690 3004255..3004617 - 363 WP_003900543.1 hypothetical protein -
BJM03_RS14695 3004620..3005747 - 1128 WP_003899420.1 site-specific integrase -
BJM03_RS14700 3005892..3006119 - 228 WP_003899421.1 hypothetical protein -
BJM03_RS14705 3006116..3006505 - 390 WP_003899422.1 hypothetical protein -
BJM03_RS14710 3006411..3006683 + 273 WP_003900544.1 hypothetical protein -
BJM03_RS14715 3006782..3007015 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2996838..3005747 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-114)

Antitoxin

Helix-turn-helix domain, group 17

(85-133)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T67725 WP_003899414.1 NZ_CP017597:c3001838-3001515 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T67725 NZ_CP017597:c3001838-3001515 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT67725 WP_003899415.1 NZ_CP017597:c3002163-3001918 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT67725 NZ_CP017597:c3002163-3001918 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References