Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2220463..2221331 Replicon chromosome
Accession NZ_CP017597
Organism Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag BJM03_RS10680 Protein ID WP_010886136.1
Coordinates 2220463..2220840 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag BJM03_RS10685 Protein ID WP_003409886.1
Coordinates 2220882..2221331 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM03_RS10630 2216252..2216704 - 453 WP_003899095.1 lipoprotein -
BJM03_RS10635 2216768..2217169 + 402 WP_003409869.1 hypothetical protein -
BJM03_RS10640 2217162..2217344 - 183 WP_003409870.1 hypothetical protein -
BJM03_RS10645 2217458..2217808 - 351 WP_003409871.1 hypothetical protein -
BJM03_RS10650 2217819..2218721 - 903 WP_003899097.1 hypothetical protein -
BJM03_RS10655 2218742..2218933 - 192 WP_003409876.1 hypothetical protein -
BJM03_RS10660 2218934..2219230 - 297 WP_003409877.1 hypothetical protein -
BJM03_RS10665 2219470..2219685 + 216 WP_003409878.1 antitoxin -
BJM03_RS10670 2219682..2219993 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
BJM03_RS22050 2219967..2220488 - 522 WP_003904745.1 hypothetical protein -
BJM03_RS10680 2220463..2220840 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
BJM03_RS10685 2220882..2221331 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
BJM03_RS10690 2221328..2221873 + 546 WP_003409891.1 SecB-like chaperone -
BJM03_RS10695 2221762..2222376 - 615 WP_003901296.1 hypothetical protein -
BJM03_RS10700 2222425..2222721 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
BJM03_RS10705 2222718..2222969 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
BJM03_RS10710 2222956..2223450 + 495 WP_003899099.1 hypothetical protein -
BJM03_RS10715 2223610..2224017 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
BJM03_RS10720 2224021..2224293 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BJM03_RS10725 2224326..2225546 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T67709 WP_010886136.1 NZ_CP017597:2220463-2220840 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T67709 NZ_CP017597:2220463-2220840 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT67709 WP_003409886.1 NZ_CP017597:2220882-2221331 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT67709 NZ_CP017597:2220882-2221331 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References