Detailed information of TA system TA067075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1458172..1458808
• Replicon: chromosome
• Accession: NZ_CP017507
• Organism: Mannheimia haemolytica strain 28253

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A378NBU4
• Locus tag: BG566_RS07280
• Protein ID: WP_032844503.1
• Coordinates: 1458172..1458477 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BG566_RS07285
• Protein ID: WP_032844505.1
• Coordinates: 1458488..1458808 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG566_RS07240	1454427..1454924	-	498	WP_020824116.1	hypothetical protein	-
BG566_RS13335	1455314..1455547	-	234	WP_061887059.1	hypothetical protein	-
BG566_RS07255	1455492..1455842	-	351	WP_006252685.1	DUF2570 domain-containing protein	-
BG566_RS07260	1455843..1456439	-	597	WP_020824120.1	glycoside hydrolase family 19 protein	-
BG566_RS07265	1456454..1456813	-	360	WP_165876904.1	phage holin, lambda family	-
BG566_RS07270	1456949..1457422	-	474	WP_006251969.1	hypothetical protein	-
BG566_RS07275	1457412..1457981	-	570	WP_032844502.1	recombination protein NinG	-
BG566_RS07280	1458172..1458477	+	306	WP_032844503.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG566_RS07285	1458488..1458808	+	321	WP_032844505.1	HigA family addiction module antidote protein	Antitoxin
BG566_RS07290	1459109..1459639	+	531	WP_032844506.1	Bro-N domain-containing protein	-
BG566_RS07295	1459795..1460223	-	429	WP_032844507.1	recombination protein NinB	-
BG566_RS07300	1460210..1460422	-	213	WP_006252693.1	hypothetical protein	-
BG566_RS07305	1460513..1461049	-	537	WP_006250086.1	DNA methyltransferase	-
BG566_RS07310	1461046..1461693	-	648	WP_032844508.1	bacteriophage replication protein	-
BG566_RS07315	1461693..1461977	-	285	WP_032844512.1	hypothetical protein	-
BG566_RS07320	1462038..1462478	-	441	Protein_1408	hypothetical protein	-
BG566_RS07325	1462475..1463314	-	840	WP_006252350.1	Rha family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1408675..1491271	82596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11980.60 Da
• Isoelectric Point: 7.1990

>T67075 WP_032844503.1 NZ_CP017507:1458172-1458477 [Mannheimia haemolytica]
MFNLTEAHFRDKALYRFFQYGEVSRTIPANLTSVLARKLDMINAAENLNDLRSPPANRLELLEPKQNNVYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL

Nucleotide

• Length: 306 bp

>T67075 NZ_CP017507:1458172-1458477 [Mannheimia haemolytica]
ATGTTTAACTTAACCGAAGCCCATTTTAGAGATAAAGCTCTTTATCGGTTCTTCCAATATGGCGAAGTCAGCAGAACAAT
CCCTGCTAATCTCACAAGCGTATTAGCTCGCAAACTGGATATGATTAATGCAGCTGAAAATCTGAATGACTTACGCAGTC
CGCCTGCTAATCGCTTAGAGTTACTTGAGCCGAAACAAAACAACGTCTATTCAATTCGAGTAAACAAACAGTATCGATTG
ATTTTCAAGTTTGAAAACAATGAACTCTCTGATTTATACCTTGACCCACACAATTACGACCTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12123.90 Da
• Isoelectric Point: 6.2139

>AT67075 WP_032844505.1 NZ_CP017507:1458488-1458808 [Mannheimia haemolytica]
MMQRKPTPIGEILQEEFLEPLNLKIGDLAEILAVHRNTASNIVNNSCRVSPEMAFKLAKAFDTSPEFWLNLQTACDLWEL
RHNNRFQQSLDSVKAVNHWQGMPAFA

Nucleotide

• Length: 321 bp

>AT67075 NZ_CP017507:1458488-1458808 [Mannheimia haemolytica]
ATGATGCAACGTAAACCCACCCCTATCGGGGAAATTCTCCAAGAAGAATTTTTAGAGCCACTTAACCTAAAAATTGGCGA
TCTTGCTGAAATCTTAGCTGTTCATCGCAATACCGCCAGCAACATTGTGAATAACAGTTGCCGTGTTAGCCCTGAAATGG
CGTTCAAACTGGCAAAAGCCTTTGATACCAGCCCTGAATTTTGGCTTAACTTACAAACTGCCTGCGATTTATGGGAACTC
CGCCATAACAACCGCTTCCAACAAAGCCTTGATAGCGTAAAAGCCGTAAATCACTGGCAAGGTATGCCTGCATTTGCTTA
A

Structures

Toxin

• AlphaFold DB: A0A378NBU4