Detailed information of TA system TA066919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1458189..1458825
• Replicon: chromosome
• Accession: NZ_CP017485
• Organism: Mannheimia haemolytica strain 39409

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A378NBU4
• Locus tag: BG571_RS07270
• Protein ID: WP_032844503.1
• Coordinates: 1458189..1458494 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BG571_RS07275
• Protein ID: WP_032844505.1
• Coordinates: 1458505..1458825 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG571_RS07230	1454444..1454941	-	498	WP_020824116.1	hypothetical protein	-
BG571_RS13325	1455331..1455564	-	234	WP_061887059.1	hypothetical protein	-
BG571_RS07245	1455509..1455859	-	351	WP_006252685.1	DUF2570 domain-containing protein	-
BG571_RS07250	1455860..1456456	-	597	WP_020824120.1	glycoside hydrolase family 19 protein	-
BG571_RS07255	1456471..1456830	-	360	WP_165876904.1	phage holin, lambda family	-
BG571_RS07260	1456966..1457439	-	474	WP_006251969.1	hypothetical protein	-
BG571_RS07265	1457429..1457998	-	570	WP_032844502.1	recombination protein NinG	-
BG571_RS07270	1458189..1458494	+	306	WP_032844503.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG571_RS07275	1458505..1458825	+	321	WP_032844505.1	HigA family addiction module antidote protein	Antitoxin
BG571_RS07280	1459126..1459656	+	531	WP_032844506.1	Bro-N domain-containing protein	-
BG571_RS07285	1459812..1460240	-	429	WP_032844507.1	recombination protein NinB	-
BG571_RS07290	1460227..1460439	-	213	WP_006252693.1	hypothetical protein	-
BG571_RS07295	1460530..1461066	-	537	WP_006250086.1	DNA methyltransferase	-
BG571_RS07300	1461063..1461710	-	648	WP_032844508.1	bacteriophage replication protein	-
BG571_RS07305	1461710..1461994	-	285	WP_032844512.1	hypothetical protein	-
BG571_RS07310	1462055..1462495	-	441	Protein_1407	hypothetical protein	-
BG571_RS07315	1462492..1463331	-	840	WP_006252350.1	Rha family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1422909..1491288	68379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11980.60 Da
• Isoelectric Point: 7.1990

>T66919 WP_032844503.1 NZ_CP017485:1458189-1458494 [Mannheimia haemolytica]
MFNLTEAHFRDKALYRFFQYGEVSRTIPANLTSVLARKLDMINAAENLNDLRSPPANRLELLEPKQNNVYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL

Nucleotide

• Length: 306 bp

>T66919 NZ_CP017485:1458189-1458494 [Mannheimia haemolytica]
ATGTTTAACTTAACCGAAGCCCATTTTAGAGATAAAGCTCTTTATCGGTTCTTCCAATATGGCGAAGTCAGCAGAACAAT
CCCTGCTAATCTCACAAGCGTATTAGCTCGCAAACTGGATATGATTAATGCAGCTGAAAATCTGAATGACTTACGCAGTC
CGCCTGCTAATCGCTTAGAGTTACTTGAGCCGAAACAAAACAACGTCTATTCAATTCGAGTAAACAAACAGTATCGATTG
ATTTTCAAGTTTGAAAACAATGAACTCTCTGATTTATACCTTGACCCACACAATTACGACCTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12123.90 Da
• Isoelectric Point: 6.2139

>AT66919 WP_032844505.1 NZ_CP017485:1458505-1458825 [Mannheimia haemolytica]
MMQRKPTPIGEILQEEFLEPLNLKIGDLAEILAVHRNTASNIVNNSCRVSPEMAFKLAKAFDTSPEFWLNLQTACDLWEL
RHNNRFQQSLDSVKAVNHWQGMPAFA

Nucleotide

• Length: 321 bp

>AT66919 NZ_CP017485:1458505-1458825 [Mannheimia haemolytica]
ATGATGCAACGTAAACCCACCCCTATCGGGGAAATTCTCCAAGAAGAATTTTTAGAGCCACTTAACCTAAAAATTGGCGA
TCTTGCTGAAATCTTAGCTGTTCATCGCAATACCGCCAGCAACATTGTGAATAACAGTTGCCGTGTTAGCCCTGAAATGG
CGTTCAAACTGGCAAAAGCCTTTGATACCAGCCCTGAATTTTGGCTTAACTTACAAACTGCCTGCGATTTATGGGAACTC
CGCCATAACAACCGCTTCCAACAAAGCCTTGATAGCGTAAAAGCCGTAAATCACTGGCAAGGTATGCCTGCATTTGCTTA
A

Structures

Toxin

• AlphaFold DB: A0A378NBU4