Detailed information of TA system TA066903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4227493..4228217
• Replicon: chromosome
• Accession: NZ_CP017482
• Organism: Pectobacterium polaris strain NIBIO1392

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: BJK05_RS19205
• Protein ID: WP_039482109.1
• Coordinates: 4227493..4227804 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BJK05_RS19210
• Protein ID: WP_095701368.1
• Coordinates: 4227801..4228217 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJK05_RS19185	4222882..4223616	+	735	WP_095701366.1	type I-E CRISPR-associated protein Cas5/CasD	-
BJK05_RS19190	4223616..4224263	+	648	WP_095701367.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
BJK05_RS19195	4224291..4225208	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
BJK05_RS19200	4225209..4225502	+	294	WP_039482111.1	type I-E CRISPR-associated endoribonuclease Cas2	-
BJK05_RS19205	4227493..4227804	+	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
BJK05_RS19210	4227801..4228217	+	417	WP_095701368.1	helix-turn-helix domain-containing protein	Antitoxin
BJK05_RS19215	4228445..4228948	-	504	WP_095701369.1	hypothetical protein	-
BJK05_RS19220	4229402..4229524	+	123	WP_125460704.1	hypothetical protein	-
BJK05_RS19225	4231856..4232314	+	459	WP_010286715.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T66903 WP_039482109.1 NZ_CP017482:4227493-4227804 [Pectobacterium polaris]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T66903 NZ_CP017482:4227493-4227804 [Pectobacterium polaris]
ATGCATGTTATTTCGCGGGCACCTTTCGACACTGCAACCACTCAGTTTCCGAATCAGGCGGCAGCACTTGATGACCTATA
TCGGGTTATCAAACGCGAAATGTATGCAACGCCGGACGATATGAAAAAACGCTTCCCCAGTCTAGATAGGATGAAATATC
GGGAAAAATGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGAGTGATGTTTTTTGCTGATTTTGAGCGAGGGAAAATC
TTCATCAAGCACATCACATCCCATGCAGAGTACGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15421.70 Da
• Isoelectric Point: 4.8037

>AT66903 WP_095701368.1 NZ_CP017482:4227801-4228217 [Pectobacterium polaris]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFESEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT66903 NZ_CP017482:4227801-4228217 [Pectobacterium polaris]
ATGATGTACGCAGACGCCATCAAGGCAGCTAACAACCTGACGAGTATCGTACCATTCCTCGGAGGCAGCACCTCTCGTAA
GGATTATGAGGATGCGCTGAAACTAACCGAATACCTGATCGAGAGTGAACCTGACAGTCCGCTAATCGATATGCTGACTG
CCAAAATTGACAAATACGAAGATGATGCGCCGGAGTTTGCAGCCTTCAACGAACGTGTTAAAGCACTCCCAGCCGGTATC
GCTTTACTGCGCGTCCTTATGGATCAGCATCAGCTAACACAGAGTGATTTTGAATCTGAGATTGGTAAAAAGTCTCTGGT
ATCCCGCATTCTAAAAGGTGAGCGCACTCTAACGCTGGATCACATGAGAGCGCTTGCTAAACGATTCAATATTCCCGTGA
GTGCTTTTGTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A093VC08