Detailed information of TA system TA066868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4828649..4829251
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: GRI03_RS23775
• Protein ID: WP_013099360.1
• Coordinates: 4828940..4829251 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GRI03_RS23770
• Protein ID: WP_013099359.1
• Coordinates: 4828649..4828939 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS23755	4826146..4827048	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
GRI03_RS23760	4827045..4827680	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
GRI03_RS23765	4827677..4828606	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
GRI03_RS23770	4828649..4828939	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
GRI03_RS23775	4828940..4829251	-	312	WP_013099360.1	hypothetical protein	Toxin
GRI03_RS23780	4829481..4830398	+	918	WP_095908404.1	alpha/beta hydrolase	-
GRI03_RS23785	4830411..4831352	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
GRI03_RS23790	4831397..4831834	-	438	WP_013099363.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GRI03_RS23795	4831831..4832712	-	882	WP_013099364.1	virulence factor BrkB family protein	-
GRI03_RS23800	4832706..4833305	-	600	WP_013099365.1	glucose-1-phosphatase	-
GRI03_RS23805	4833425..4834225	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T66868 WP_013099360.1 NZ_CP017475:c4829251-4828940 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T66868 NZ_CP017475:c4829251-4828940 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAGTTTGAAATCAGATTGCTGCTCATCTAC
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT66868 WP_013099359.1 NZ_CP017475:c4828939-4828649 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT66868 NZ_CP017475:c4828939-4828649 [Enterobacter cloacae]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGGCGTTCACCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAATGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02