Detailed information of TA system TA066864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3083443..3084103
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1ABU6
• Locus tag: GRI03_RS15455
• Protein ID: WP_020687566.1
• Coordinates: 3083729..3084103 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GRI03_RS15450
• Protein ID: WP_014832568.1
• Coordinates: 3083443..3083745 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS15430	3079367..3081556	+	2190	WP_013097826.1	TonB-dependent siderophore receptor	-
GRI03_RS15440	3081884..3082273	-	390	WP_095908000.1	RidA family protein	-
GRI03_RS15445	3082415..3083347	+	933	WP_095908001.1	LysR family transcriptional regulator	-
GRI03_RS15450	3083443..3083745	-	303	WP_014832568.1	XRE family transcriptional regulator	Antitoxin
GRI03_RS15455	3083729..3084103	-	375	WP_020687566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GRI03_RS15460	3084462..3085412	-	951	WP_013097829.1	HTH-type transcriptional regulator Cbl	-
GRI03_RS15465	3085509..3086426	-	918	WP_010432103.1	nitrogen assimilation transcriptional regulator NAC	-
GRI03_RS15475	3086908..3087843	-	936	WP_095908002.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14330.32 Da
• Isoelectric Point: 9.7082

>T66864 WP_020687566.1 NZ_CP017475:c3084103-3083729 [Enterobacter cloacae]
VWTINTTDRFDCWFRSLDDTDRASVLAALLVLREKGPALSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Nucleotide

• Length: 375 bp

>T66864 NZ_CP017475:c3084103-3083729 [Enterobacter cloacae]
GTGTGGACAATTAACACGACAGACAGGTTCGATTGCTGGTTTCGTTCACTTGACGATACTGATCGGGCCAGCGTGCTGGC
AGCACTGCTTGTTTTGCGAGAAAAGGGACCTGCTTTGTCGAGGCCCTATGCCGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTGCGTATTCAGAGTAAGGGCGATCCGTTGCGGGCATTTTTCGCGTTTGACCCAAACCGTACCGGCATT
GTGCTCTGTGCCGGCAATAAGGTCGGCAACGAAAGACGCTTTTACGATGAAATGCTTCTGGTGGCCGACCGGGAATTCAC
TCACTGGTTGAATACATTAAAAGAGAGGACGTTACGCGATGGGCAGAAACCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10972.69 Da
• Isoelectric Point: 5.1473

>AT66864 WP_014832568.1 NZ_CP017475:c3083745-3083443 [Enterobacter cloacae]
MGRNLEQLIADEKSDVVASAQAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLTLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT66864 NZ_CP017475:c3083745-3083443 [Enterobacter cloacae]
ATGGGCAGAAACCTTGAGCAGCTTATAGCGGATGAAAAATCGGATGTTGTTGCCAGCGCTCAGGCAATGGCTGCCGACAT
TTTGCTTAATATCCATCTGGCAGAGCTACGTGAAAAGGTGCAAAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGCATCC
GACAGCCCACCGTCGCGGTCATGGAAAAGCCCGGGCGCGATCTGAAACTCTCTTCACTCAAGCGCTACGTTGAGGCTGCG
GGTGGAAAGCTTACGCTTGATGTTGAGTTACCGGATGGTTCGCATTATGAGTTTGTGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A5E1ABU6