Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1218730..1219379 Replicon chromosome
Accession NZ_CP017475
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag GRI03_RS06585 Protein ID WP_013097692.1
Coordinates 1219017..1219379 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag GRI03_RS06580 Protein ID WP_013097693.1
Coordinates 1218730..1219029 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS06570 1215142..1217115 + 1974 WP_057071947.1 PhoX family phosphatase -
GRI03_RS06575 1217218..1218606 + 1389 WP_023620421.1 phenylalanine transporter -
GRI03_RS06580 1218730..1219029 - 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
GRI03_RS06585 1219017..1219379 - 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GRI03_RS06590 1219585..1220823 + 1239 WP_029882613.1 MFS transporter -
GRI03_RS06595 1220839..1221864 + 1026 WP_095907479.1 sugar phosphate isomerase/epimerase -
GRI03_RS06600 1221857..1222999 + 1143 WP_095907480.1 Gfo/Idh/MocA family oxidoreductase -
GRI03_RS06605 1223075..1224046 + 972 WP_023620419.1 LacI family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T66855 WP_013097692.1 NZ_CP017475:c1219379-1219017 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T66855 NZ_CP017475:c1219379-1219017 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT66855 WP_013097693.1 NZ_CP017475:c1219029-1218730 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT66855 NZ_CP017475:c1219029-1218730 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTAATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACAGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTGACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References