Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 97799..98445 Replicon chromosome
Accession NZ_CP017475
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag GRI03_RS01390 Protein ID WP_095908420.1
Coordinates 98095..98445 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GRI03_RS01385 Protein ID WP_095907190.1
Coordinates 97799..98098 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS01360 93757..94548 + 792 WP_029881882.1 winged helix-turn-helix domain-containing protein -
GRI03_RS01365 94550..95032 + 483 WP_013094867.1 hypothetical protein -
GRI03_RS01370 95271..96158 + 888 WP_095907187.1 helix-turn-helix domain-containing protein -
GRI03_RS01375 96244..97242 + 999 WP_095907188.1 class I SAM-dependent methyltransferase -
GRI03_RS01380 97332..97769 + 438 WP_095907189.1 acetyltransferase -
GRI03_RS01385 97799..98098 - 300 WP_095907190.1 XRE family transcriptional regulator Antitoxin
GRI03_RS01390 98095..98445 - 351 WP_095908420.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GRI03_RS01395 98690..98926 - 237 WP_095907191.1 SymE family type I addiction module toxin -
GRI03_RS01400 99018..99209 + 192 WP_028028019.1 toxin-antitoxin system HicB family antitoxin -
GRI03_RS01405 99252..99545 - 294 WP_020686479.1 hypothetical protein -
GRI03_RS01410 99553..99981 - 429 WP_185868267.1 hypothetical protein -
GRI03_RS01420 100672..102990 + 2319 WP_095907193.1 alpha-xylosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 103110..104252 1142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13451.54 Da        Isoelectric Point: 8.0096

>T66850 WP_095908420.1 NZ_CP017475:c98445-98095 [Enterobacter cloacae]
MWTVLFGKVFEQWLLEQEEGLQDKVLADLLNLQNYGPRLPRPYADTVKGSRYKHMKELRIQYAGRPIRAFFAFDPVRQAI
VLCAGDKSNDNVFYEKMIRIADAEFSLHLTSLEAAK

Download         Length: 351 bp

>T66850 NZ_CP017475:c98445-98095 [Enterobacter cloacae]
ATGTGGACGGTTCTGTTCGGAAAGGTATTTGAGCAATGGTTGCTTGAACAGGAAGAAGGATTACAGGATAAGGTACTGGC
AGACCTGCTAAATCTGCAAAACTATGGGCCACGTCTTCCTCGTCCCTATGCAGATACGGTTAAAGGCTCACGGTATAAGC
ATATGAAAGAATTACGTATTCAGTACGCAGGTCGGCCGATACGTGCGTTCTTCGCATTTGATCCTGTCCGTCAGGCTATT
GTGCTTTGCGCGGGCGATAAGAGTAACGACAACGTCTTTTATGAAAAAATGATTCGCATCGCAGATGCCGAATTTTCCCT
TCATCTGACAAGCCTGGAGGCAGCCAAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11144.96 Da        Isoelectric Point: 7.0164

>AT66850 WP_095907190.1 NZ_CP017475:c98098-97799 [Enterobacter cloacae]
VKTLNDVIASRSPESQQRIKEMAEEMILETGLQLLREELKLSQKNVADLMGVSQPAVTQLEQRGNELKITTLKRYIEAMG
GKLRIDVELPTGKHVAFQI

Download         Length: 300 bp

>AT66850 NZ_CP017475:c98098-97799 [Enterobacter cloacae]
GTGAAAACCTTAAATGATGTGATTGCGTCACGTTCACCGGAAAGCCAACAACGTATTAAAGAGATGGCAGAGGAAATGAT
CCTCGAAACGGGTCTGCAACTTTTACGTGAAGAGCTCAAGCTTTCGCAAAAAAATGTTGCCGACCTGATGGGGGTGAGCC
AACCCGCAGTAACGCAGCTAGAGCAACGTGGAAATGAGCTAAAAATTACCACGCTAAAGCGCTACATTGAGGCTATGGGT
GGGAAACTCCGTATCGACGTTGAACTTCCCACCGGAAAACACGTTGCATTTCAAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References