Detailed information of TA system TA066849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31659..32389
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Z1J9A0
• Locus tag: GRI03_RS01055
• Protein ID: WP_020684666.1
• Coordinates: 31659..31973 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GRI03_RS01060
• Protein ID: WP_020684665.1
• Coordinates: 31970..32389 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS01030	27323..28411	+	1089	WP_032618344.1	cellulase family glycosylhydrolase	-
GRI03_RS01035	28314..29498	-	1185	WP_023620624.1	multidrug efflux MFS transporter EmrD	-
GRI03_RS01040	29674..30507	-	834	WP_095907177.1	DMT family transporter	-
GRI03_RS01045	30570..31016	-	447	WP_013094803.1	GNAT family N-acetyltransferase	-
GRI03_RS01050	31081..31170	-	90	WP_020684667.1	type I toxin-antitoxin system toxin TisB	-
GRI03_RS01055	31659..31973	+	315	WP_020684666.1	type II toxin-antitoxin system HigB family toxin	Toxin
GRI03_RS01060	31970..32389	+	420	WP_020684665.1	helix-turn-helix domain-containing protein	Antitoxin
GRI03_RS01065	32536..32634	+	99	WP_071830013.1	ilvB operon leader peptide IvbL	-
GRI03_RS01070	32798..34486	+	1689	WP_048972063.1	acetolactate synthase large subunit	-
GRI03_RS01075	34490..34777	+	288	WP_001171659.1	acetolactate synthase small subunit	-
GRI03_RS01080	34860..35453	+	594	WP_058650559.1	transcriptional regulator UhpA	-
GRI03_RS01085	35450..36955	+	1506	WP_013094810.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12367.51 Da
• Isoelectric Point: 10.2509

>T66849 WP_020684666.1 NZ_CP017475:31659-31973 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLVLGRTLEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T66849 NZ_CP017475:31659-31973 [Enterobacter cloacae]
ATGCATTTAATCTCGATGAAGGCAATTTTGGATGCGGTTATCCAATTTCCGCAGCACAGGGAAGAACTCCTCGTTTTGGG
ACGCACTCTTGAAAAGAGTCATTGCCCAACACCTGCTGCGTTAAAAAAACTATTTCCGACGCTGGATAACTTCAAGTATC
TGGATAAGCATTATGTTATTGATATTGCGAACAACAATCTTAGGGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAAAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15569.13 Da
• Isoelectric Point: 4.9597

>AT66849 WP_020684665.1 NZ_CP017475:31970-32389 [Enterobacter cloacae]
MMIVADAMKATHALVEAVPLLGEQPSEKDYNDALELVEYLLINEPNSPLLDIVCARISRYEANRPEIVALRKEMELVPVG
IAVLRTLMDQYKLTTSDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLASRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT66849 NZ_CP017475:31970-32389 [Enterobacter cloacae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGAGGCCGTTCCTCTATTGGGAGAGCAGCCGAGCGA
GAAAGATTATAACGATGCACTGGAGTTGGTTGAGTATCTGCTTATTAACGAACCTAATAGCCCATTGCTGGATATCGTGT
GCGCCAGAATCAGCCGTTACGAAGCCAATCGGCCAGAGATCGTCGCGTTACGCAAGGAGATGGAACTCGTGCCTGTCGGT
ATCGCGGTTTTAAGAACCTTGATGGATCAGTATAAATTGACAACATCTGATTTTCAGGATGAAATTGGTAGTAAATCAAT
GGTTTCAAGGGTTCTGAATGGTCAGCGACAACTTACTCTGAACCACATTAAAAAGCTGGCGTCCAGATTTGGTGTTTCAC
CAGCACTATTTATTGAATAA

Structures

Toxin

• AlphaFold DB: A0A7Z1J9A0