Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 124591..125093 Replicon plasmid pM12X01451
Accession NZ_CP017473
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag GRI03_RS00650 Protein ID WP_095907130.1
Coordinates 124839..125093 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag GRI03_RS00645 Protein ID WP_095907129.1
Coordinates 124591..124842 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS00610 119764..120219 + 456 WP_095907124.1 transglycosylase SLT domain-containing protein -
GRI03_RS00615 120231..120770 - 540 WP_095907171.1 antirestriction protein -
GRI03_RS00620 121309..122139 - 831 WP_095907125.1 type I restriction-modification system subunit M -
GRI03_RS00625 122185..122451 - 267 WP_095907126.1 hypothetical protein -
GRI03_RS00630 122537..122881 - 345 WP_095907127.1 hypothetical protein -
GRI03_RS00635 122932..123300 - 369 WP_095907128.1 hypothetical protein -
GRI03_RS00640 123517..124494 + 978 WP_095907172.1 IS110 family transposase -
GRI03_RS00645 124591..124842 + 252 WP_095907129.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GRI03_RS00650 124839..125093 + 255 WP_095907130.1 Txe/YoeB family addiction module toxin Toxin
GRI03_RS24195 125567..125725 - 159 WP_187143871.1 hypothetical protein -
GRI03_RS00660 126017..126517 - 501 WP_095907131.1 antirestriction protein ArdA -
GRI03_RS00665 126560..126985 - 426 WP_095907132.1 antirestriction protein -
GRI03_RS00670 127247..127552 + 306 WP_095907133.1 chorismate mutase -
GRI03_RS00675 128016..128246 - 231 WP_095907134.1 hypothetical protein -
GRI03_RS00680 128286..128904 - 619 Protein_134 Mediator of plasmid stability -
GRI03_RS00685 128907..129227 - 321 Protein_135 StbA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..169226 169226
- flank IS/Tn - - 123514..124494 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10170.61 Da        Isoelectric Point: 8.8560

>T66847 WP_095907130.1 NZ_CP017473:124839-125093 [Enterobacter cloacae]
VKLTWSEEAWDDYLYWQETDKRMVKKINELIKDIRRTPFDGKGKPEPLKHNLSGFWSRRITEEHRLVYAITADTIQIAAC
RYHY

Download         Length: 255 bp

>T66847 NZ_CP017473:124839-125093 [Enterobacter cloacae]
GTGAAGCTCACCTGGTCTGAAGAAGCCTGGGATGATTATCTTTATTGGCAGGAAACAGATAAGCGTATGGTCAAAAAGAT
CAACGAGTTAATCAAAGATATTCGCAGAACGCCCTTTGATGGCAAGGGGAAACCTGAGCCATTAAAACACAACCTGTCAG
GTTTTTGGTCGCGCCGCATAACTGAGGAACATCGACTGGTATATGCAATTACCGCCGACACCATCCAGATAGCAGCTTGT
CGTTATCACTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9303.52 Da        Isoelectric Point: 4.8090

>AT66847 WP_095907129.1 NZ_CP017473:124591-124842 [Enterobacter cloacae]
MRTISYSEARQNLSATMVKTVEDHAPILITRQNGESCVLMSLEEYNSLEETAYLLRSPANARRLSDSIESLKSGKGIEKD
IIE

Download         Length: 252 bp

>AT66847 NZ_CP017473:124591-124842 [Enterobacter cloacae]
ATGCGCACAATTAGTTATAGCGAAGCCCGGCAGAATTTATCAGCAACTATGGTGAAAACTGTAGAGGATCATGCCCCAAT
TTTGATCACCCGACAGAACGGGGAATCATGCGTTCTTATGTCACTTGAAGAATATAACTCGCTGGAAGAAACAGCTTATC
TTCTACGTTCACCTGCAAATGCCAGAAGGCTCTCGGATTCAATTGAAAGTCTGAAATCTGGGAAAGGTATTGAAAAGGAT
ATCATCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References