Detailed information of TA system TA066806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 100154..100848
• Replicon: chromosome
• Accession: NZ_CP017454
• Organism: Dickeya solani strain PPO 9019

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: BJJ98_RS00445
• Protein ID: WP_026594479.1
• Coordinates: 100450..100848 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A2K8VUD1
• Locus tag: BJJ98_RS00440
• Protein ID: WP_022633740.1
• Coordinates: 100154..100447 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ98_RS00425	95863..97176	+	1314	WP_022633743.1	lytic murein transglycosylase	-
BJJ98_RS00430	97187..98347	-	1161	WP_022633742.1	alpha/beta fold hydrolase	-
BJJ98_RS00435	98887..99936	+	1050	WP_022633741.1	hypothetical protein	-
BJJ98_RS00440	100154..100447	+	294	WP_022633740.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BJJ98_RS00445	100450..100848	+	399	WP_026594479.1	type II toxin-antitoxin system YafO family toxin	Toxin
BJJ98_RS00450	101133..101360	+	228	WP_022633738.1	type III secretion protein HrpF	-
BJJ98_RS00455	101347..101781	+	435	WP_022633737.1	hypothetical protein	-
BJJ98_RS00460	101774..103843	+	2070	WP_023637836.1	type III secretion system outer membrane ring subunit SctC	-
BJJ98_RS00465	103878..104066	+	189	WP_022633734.1	hypothetical protein	-
BJJ98_RS00470	104063..104473	+	411	WP_022633733.1	hypothetical protein	-
BJJ98_RS00475	104634..105668	+	1035	WP_022633732.1	harpin HrpZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15230.56 Da
• Isoelectric Point: 8.4931

>T66806 WP_026594479.1 NZ_CP017454:100450-100848 [Dickeya solani]
IRIFKSTLIRQQLSQQELDDLVADFHAYKQNGVLPDTFGRDAPYDDDRTYPLVKAEQVAHIHLADADAPFPRFLRQFKRT
SDRAHLVYCQGAMDPEACLLIIILKPEAHKMARNNNNMHKIGMMAAAFRMKY

Nucleotide

• Length: 399 bp

>T66806 NZ_CP017454:100450-100848 [Dickeya solani]
ATACGGATTTTCAAGTCAACGCTGATCCGTCAACAATTGAGCCAGCAAGAGCTTGATGATTTGGTCGCGGATTTTCACGC
CTATAAGCAGAATGGGGTACTACCGGACACATTTGGGCGTGACGCGCCTTATGATGATGACCGAACCTATCCATTAGTAA
AAGCAGAGCAGGTGGCTCACATCCACCTTGCCGATGCGGATGCGCCTTTTCCCAGGTTCTTGCGCCAGTTCAAGAGAACC
AGCGATCGGGCCCATTTGGTCTACTGCCAGGGAGCGATGGATCCCGAGGCCTGCCTGTTAATTATTATTTTAAAACCCGA
AGCCCATAAAATGGCACGCAACAATAACAACATGCACAAGATCGGGATGATGGCCGCTGCGTTCAGGATGAAATATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10934.48 Da
• Isoelectric Point: 5.2082

>AT66806 WP_022633740.1 NZ_CP017454:100154-100447 [Dickeya solani]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEAKQETVHTVARFRPTAERLRALADSG
QKLLNDASGKDLTEFTE

Nucleotide

• Length: 294 bp

>AT66806 NZ_CP017454:100154-100447 [Dickeya solani]
ATGGAGCGTATTCTTGCTGAAAAATCTATAAATATTACAGAGTTAAGGAAAAATCCGGCTAAGTATTTTATTGATGAGCC
TGTTGCCGTGCTTTCTAACAACCGGCCCGCCGGTTACATGGTGAGTGCCAAAGTCTTTGAAGAACTGATCGATCTGCTAG
AGGCGAAGCAGGAAACGGTTCATACCGTAGCGCGCTTTAGGCCAACGGCCGAACGTTTACGAGCCCTTGCAGATAGCGGT
CAGAAACTGCTGAATGATGCCTCTGGCAAGGATCTGACTGAGTTCACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8VUD1