Detailed information of TA system TA066688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4360337..4361031
• Replicon: chromosome
• Accession: NZ_CP017442
• Organism: Escherichia coli O157:H7 strain 4276

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A4C51_RS22555
• Protein ID: WP_001263495.1
• Coordinates: 4360337..4360735 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A4C51_RS22560
• Protein ID: WP_000554757.1
• Coordinates: 4360738..4361031 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4355924..4356004	-	81	NuclAT_11	-	-
-	4355924..4356004	-	81	NuclAT_11	-	-
-	4355924..4356004	-	81	NuclAT_11	-	-
-	4355924..4356004	-	81	NuclAT_11	-	-
A4C51_RS22530	4356600..4357058	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A4C51_RS22535	4357319..4358776	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A4C51_RS22540	4358833..4359354	-	522	Protein_4261	peptide chain release factor H	-
A4C51_RS22545	4359353..4359556	-	204	Protein_4262	RNA ligase RtcB family protein	-
A4C51_RS22550	4359875..4360327	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A4C51_RS22555	4360337..4360735	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A4C51_RS22560	4360738..4361031	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4C51_RS22565	4361083..4362138	-	1056	WP_001226186.1	DNA polymerase IV	-
A4C51_RS22570	4362209..4362994	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A4C51_RS22575	4362966..4364678	+	1713	Protein_4268	flagellar biosynthesis protein FlhA	-
A4C51_RS31350	4364823..4365320	-	498	Protein_4269	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T66688 WP_001263495.1 NZ_CP017442:c4360735-4360337 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66688 NZ_CP017442:c4360735-4360337 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66688 WP_000554757.1 NZ_CP017442:c4361031-4360738 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66688 NZ_CP017442:c4361031-4360738 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6