Detailed information of TA system TA066680

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2888158..2888383
• Replicon: chromosome
• Accession: NZ_CP017442
• Organism: Escherichia coli O157:H7 strain 4276

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A4C51_RS14850
• Protein ID: WP_000813258.1
• Coordinates: 2888158..2888313 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2888325..2888383 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C51_RS14800	2883160..2883588	-	429	WP_001303509.1	tellurite resistance protein	-
A4C51_RS14815	2884043..2884756	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A4C51_RS30965	2884892..2885089	-	198	WP_000917763.1	hypothetical protein	-
A4C51_RS14825	2885314..2885868	-	555	WP_000640035.1	DUF1133 family protein	-
A4C51_RS14830	2885931..2886236	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A4C51_RS14835	2886249..2887298	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
A4C51_RS14840	2887300..2887572	-	273	WP_000191872.1	hypothetical protein	-
A4C51_RS14845	2887694..2888038	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A4C51_RS14850	2888158..2888313	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2888325..2888383	+	59	-	-	Antitoxin
A4C51_RS14855	2888604..2889161	-	558	WP_000104474.1	DUF551 domain-containing protein	-
A4C51_RS14860	2889163..2889381	-	219	WP_000683609.1	DUF4014 family protein	-
A4C51_RS14865	2889509..2889820	-	312	WP_001289673.1	hypothetical protein	-
A4C51_RS14870	2889813..2890040	-	228	WP_000699809.1	hypothetical protein	-
A4C51_RS14875	2890037..2890318	-	282	WP_000603384.1	DNA-binding protein	-
A4C51_RS14880	2890351..2891067	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
A4C51_RS30970	2891101..2891562	-	462	WP_000139447.1	replication protein	-
A4C51_RS30975	2891555..2892598	-	1044	WP_001262402.1	hypothetical protein	-
A4C51_RS14895	2892667..2893092	-	426	WP_000693878.1	Rha family transcriptional regulator	-
A4C51_RS14900	2893076..2893318	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	2851066..2950465	99399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T66680 WP_000813258.1 NZ_CP017442:c2888313-2888158 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T66680 NZ_CP017442:c2888313-2888158 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT66680 NZ_CP017442:2888325-2888383 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin