Detailed information of TA system TA066621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4372403..4373097
• Replicon: chromosome
• Accession: NZ_CP017438
• Organism: Escherichia coli O157:H7 strain 2159

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A4C45_RS22650
• Protein ID: WP_001263495.1
• Coordinates: 4372403..4372801 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A4C45_RS22655
• Protein ID: WP_000554757.1
• Coordinates: 4372804..4373097 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4367990..4368070	-	81	NuclAT_11	-	-
-	4367990..4368070	-	81	NuclAT_11	-	-
-	4367990..4368070	-	81	NuclAT_11	-	-
-	4367990..4368070	-	81	NuclAT_11	-	-
A4C45_RS22625	4368666..4369124	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A4C45_RS22630	4369385..4370842	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A4C45_RS22635	4370899..4371420	-	522	Protein_4276	peptide chain release factor H	-
A4C45_RS22640	4371419..4371622	-	204	Protein_4277	RNA ligase RtcB family protein	-
A4C45_RS22645	4371941..4372393	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A4C45_RS22650	4372403..4372801	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A4C45_RS22655	4372804..4373097	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4C45_RS22660	4373149..4374204	-	1056	WP_001226186.1	DNA polymerase IV	-
A4C45_RS22665	4374275..4375060	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A4C45_RS22670	4375032..4376744	+	1713	Protein_4283	flagellar biosynthesis protein FlhA	-
A4C45_RS31450	4376889..4377386	-	498	Protein_4284	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T66621 WP_001263495.1 NZ_CP017438:c4372801-4372403 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66621 NZ_CP017438:c4372801-4372403 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66621 WP_000554757.1 NZ_CP017438:c4373097-4372804 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66621 NZ_CP017438:c4373097-4372804 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6