Detailed information of TA system TA066616

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3493281..3493506
• Replicon: chromosome
• Accession: NZ_CP017438
• Organism: Escherichia coli O157:H7 strain 2159

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A4C45_RS18435
• Protein ID: WP_000813263.1
• Coordinates: 3493281..3493436 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3493448..3493506 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C45_RS18400	3488735..3489448	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
A4C45_RS18405	3489586..3489782	-	197	Protein_3476	TrmB family transcriptional regulator	-
A4C45_RS18410	3490069..3490887	-	819	WP_000265269.1	CPBP family intramembrane metalloprotease	-
A4C45_RS18415	3491039..3491410	-	372	WP_000090264.1	antitermination protein	-
A4C45_RS18420	3491400..3491771	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A4C45_RS18425	3491784..3492833	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
A4C45_RS29800	3492835..3493113	-	279	WP_001341388.1	hypothetical protein	-
A4C45_RS18435	3493281..3493436	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3493448..3493506	+	59	-	-	Antitoxin
A4C45_RS18440	3494041..3494814	+	774	WP_000160654.1	alpha/beta hydrolase	-
A4C45_RS18445	3495166..3495579	-	414	WP_001151233.1	DUF977 family protein	-
A4C45_RS18450	3495595..3496365	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
A4C45_RS18455	3496387..3497133	-	747	WP_000788751.1	ATP-binding protein	-
A4C45_RS18460	3497140..3498231	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T66616 WP_000813263.1 NZ_CP017438:c3493436-3493281 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T66616 NZ_CP017438:c3493436-3493281 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT66616 NZ_CP017438:3493448-3493506 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin