Detailed information of TA system TA066585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4363984..4364678
• Replicon: chromosome
• Accession: NZ_CP017436
• Organism: Escherichia coli O157:H7 strain 2149

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A4C44_RS22590
• Protein ID: WP_001263495.1
• Coordinates: 4363984..4364382 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A4C44_RS22595
• Protein ID: WP_000554757.1
• Coordinates: 4364385..4364678 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4359571..4359651	-	81	NuclAT_11	-	-
-	4359571..4359651	-	81	NuclAT_11	-	-
-	4359571..4359651	-	81	NuclAT_11	-	-
-	4359571..4359651	-	81	NuclAT_11	-	-
A4C44_RS22565	4360247..4360705	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A4C44_RS22570	4360966..4362423	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A4C44_RS22575	4362480..4363001	-	522	Protein_4264	peptide chain release factor H	-
A4C44_RS22580	4363000..4363203	-	204	Protein_4265	RNA ligase RtcB family protein	-
A4C44_RS22585	4363522..4363974	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A4C44_RS22590	4363984..4364382	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A4C44_RS22595	4364385..4364678	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4C44_RS22600	4364730..4365785	-	1056	WP_001226186.1	DNA polymerase IV	-
A4C44_RS22605	4365856..4366641	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A4C44_RS22610	4366613..4368325	+	1713	Protein_4271	flagellar biosynthesis protein FlhA	-
A4C44_RS31355	4368470..4368967	-	498	Protein_4272	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T66585 WP_001263495.1 NZ_CP017436:c4364382-4363984 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66585 NZ_CP017436:c4364382-4363984 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66585 WP_000554757.1 NZ_CP017436:c4364678-4364385 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66585 NZ_CP017436:c4364678-4364385 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6