Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4726090..4726502 Replicon chromosome
Accession NZ_CP017434
Organism Escherichia coli O157:H7 strain 1130

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag A4C50_RS24320 Protein ID WP_000132630.1
Coordinates 4726161..4726502 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4726090..4726166 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C50_RS24310 4722717..4724186 + 1470 WP_070081075.1 type I restriction-modification system subunit M -
A4C50_RS24315 4724186..4725940 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- 4726090..4726166 - 77 NuclAT_14 - Antitoxin
- 4726090..4726166 - 77 NuclAT_14 - Antitoxin
- 4726090..4726166 - 77 NuclAT_14 - Antitoxin
- 4726090..4726166 - 77 NuclAT_14 - Antitoxin
- 4726090..4726166 - 77 NuclAT_15 - Antitoxin
- 4726090..4726166 - 77 NuclAT_15 - Antitoxin
- 4726090..4726166 - 77 NuclAT_15 - Antitoxin
- 4726090..4726166 - 77 NuclAT_15 - Antitoxin
A4C50_RS24320 4726161..4726502 + 342 WP_000132630.1 endoribonuclease SymE Toxin
A4C50_RS30255 4726549..4727712 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
A4C50_RS24330 4727760..4728641 - 882 WP_001304007.1 DUF262 domain-containing protein -
A4C50_RS24335 4728738..4728902 - 165 WP_000394274.1 DUF1127 domain-containing protein -
A4C50_RS24340 4729079..4730491 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -
A4C50_RS31760 4730734..4730934 - 201 WP_001310455.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 / espX6 4718289..4739498 21209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T66552 WP_000132630.1 NZ_CP017434:4726161-4726502 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T66552 NZ_CP017434:4726161-4726502 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT66552 NZ_CP017434:c4726166-4726090 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References