Detailed information of TA system TA066549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4372171..4372865
• Replicon: chromosome
• Accession: NZ_CP017434
• Organism: Escherichia coli O157:H7 strain 1130

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A4C50_RS22630
• Protein ID: WP_001263495.1
• Coordinates: 4372171..4372569 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A4C50_RS22635
• Protein ID: WP_000554757.1
• Coordinates: 4372572..4372865 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4367758..4367838	-	81	NuclAT_11	-	-
-	4367758..4367838	-	81	NuclAT_11	-	-
-	4367758..4367838	-	81	NuclAT_11	-	-
-	4367758..4367838	-	81	NuclAT_11	-	-
A4C50_RS22605	4368434..4368892	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A4C50_RS22610	4369153..4370610	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A4C50_RS22615	4370667..4371188	-	522	Protein_4276	peptide chain release factor H	-
A4C50_RS22620	4371187..4371390	-	204	Protein_4277	RNA ligase RtcB family protein	-
A4C50_RS22625	4371709..4372161	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A4C50_RS22630	4372171..4372569	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A4C50_RS22635	4372572..4372865	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4C50_RS22640	4372917..4373972	-	1056	WP_001226186.1	DNA polymerase IV	-
A4C50_RS22645	4374043..4374828	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A4C50_RS22650	4374800..4376512	+	1713	Protein_4283	flagellar biosynthesis protein FlhA	-
A4C50_RS31435	4376657..4377154	-	498	Protein_4284	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4331110..4378103	46993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T66549 WP_001263495.1 NZ_CP017434:c4372569-4372171 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66549 NZ_CP017434:c4372569-4372171 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66549 WP_000554757.1 NZ_CP017434:c4372865-4372572 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66549 NZ_CP017434:c4372865-4372572 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6